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J Hazard Mater. 2019 Jul 5;373:716-724. doi: 10.1016/j.jhazmat.2019.03.109. Epub 2019 Mar 26.

Carbon sources mediate microbial pentachlorophenol dechlorination in soils.

Author information

1
School of Computer Science, South China Normal University, Guangzhou, 510631, PR China.
2
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China.
3
Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou, 510650, PR China; College of Architecture and Environment, Sichuan University, Chengdu, 610065, PR China.
4
Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou, 510650, PR China; School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, 510006, PR China.
5
Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science & Technology, Guangzhou, 510650, PR China. Electronic address: taoliang@soil.gd.cn.

Abstract

In this study, experiments were performed using network analysis to investigate the effects of different carbon sources, including blank, citrate, glucose and lactate, on indigenous bacterial communities and on the pentachlorophenol (PCP) dechlorination in two soils. Kinetics results demonstrate that PCP dechlorination is significantly enhanced by adding citrate/lactate, but to a lesser extent by adding glucose. High-throughput sequencing results revealed that Firmicutes and Proteobacteria were the dominant groups in these four different treatments during the PCP dechlorination, whereas random forest analysis indicated that the orders Clostridiales, Haloplasmatales, Bacillales, Pseudomonadales and Gaiellales were the critical bacterial orders in modules that were significantly correlated with PCP dechlorination. Among them, the relative abundance of Clostridiales dramatically increased in both citrate and lactate treatment, further accelerating the PCP dechlorination. Addition of citrate/lactate as the carbon source increased the bacterial co-occurrence network density, average clustering coefficient and modularity. Moreover, more modules significantly correlated with PCP dechlorination in the citrate/lactate networks compared with the glucose/blank networks. Random forest modeling suggested that Clostridiales played a critical role in these functional modules. Taken together, our results provide insight into the biological mechanism of the impact of exogenous carbon sources on PCP dechlorination pathways by modifying soil bacterial networks.

KEYWORDS:

Bacterial co-occurrence network; Bacterial community composition; Carbon sources; Dechlorination; High-throughput sequencing

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