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Avian Pathol. 2019 Aug;48(4):343-351. doi: 10.1080/03079457.2019.1605145. Epub 2019 Apr 30.

Retrospective investigation and molecular characteristics of the recombinant Muscovy duck parvovirus circulating in Muscovy duck flocks in China.

Wang J1,2,3,4, Wang Z1,2, Jia J1,2, Ling J1,2, Mi Q1,2, Zhu G1,2,3,4.

Author information

1
a College of Veterinary Medicine , Yangzhou University , Yangzhou , People's Republic of China.
2
b Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonosis , Yangzhou , People's Republic of China.
3
c Jiangsu Key Laboratory of Preventive Veterinary Medicine , Yangzhou , People's Republic of China.
4
d Key Laboratory for Avian Bioproducts Development , Ministry of Agriculture , Yangzhou , Jiangsu , People's Republic of China.

Abstract

The recombinant Muscovy duck parvovirus (rMDPV) has been recently characterized and identified in China. However, whether other additional rMDPV field isolates exist, and whether these strains possess common molecular characteristics, remain to be explored. In this retrospective study, two new rMDPV isolates, namely, JH06 and JH10, were identified through genome sequencing and recombination analysis. JH06, JH10, and four previously characterized rMDPV strains (SAAS-SHNH, ZW, FJM3, and PT97) underwent the same recombination events in a 1.1-kb region in their VP3 genes and displayed highly consistent beginning and ending breakpoints. JH06, JH10, SAAS-SHNH, ZW, and FJM3, but not PT97, underwent recombination in their P9 promoter regions. In both recombination events, the classical MDPV strain YY acted as the major parent, whereas the virulent strain DY16 and the vaccine strain SYG61v of goose parvovirus (GPV) served as the minor parents. The sequence alignments of inverted terminal repeats (ITRs) revealed that rMDPV strains shared higher identities (96.0%-97.2%) with classical MDPV strains than with GPV and contained typical one-nucleotide-pair deletions in the palindromic stems of their ITRs. This work elucidated the common molecular characteristics and differences of six rMDPV strains. The results of this work will facilitate the preparation of an efficacious vaccine for the protection of Muscovy ducks against rMDPV infection.

KEYWORDS:

Muscovy duck parvovirus; P9 promoter; goose parvovirus; inverted terminal repeats; phylogenetic tree; recombination

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