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Biomed Res Int. 2019 Mar 7;2019:3746326. doi: 10.1155/2019/3746326. eCollection 2019.

Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property.

Li T1, Zhu J1, Deng F1, Wu W1, Zheng Z1, Lv C1, Li Y1, Xiang W2, Lu X1,2, Qin S1,2,3.

Author information

1
Core Research Program 1515, Key Laboratory for Food Science and Biotechnology of Hunan Province, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
2
Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
3
The United Graduate School of Agricultural Sciences, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan.

Abstract

Myricetin has been reported as a promising chemopreventive compound with multiple biofunctions. To evaluate its influence on gene expressions in genome-wide set and further investigate its anti-inflammatory property, the present study performed Gene Ontology and Ingenuity Pathway Analysis (IPA) to describe the basic gene expression characteristics by myricetin treatment in HepG2 cells, confirmed its multi-biofunction by real-time fluorescent quantitative PCR (RT-qPCR), and further verified its anti-inflammatory property by Western blotting and bio-plex-based cytokines assay. The IPA data showed that 337 gene expressions (48% of the top molecules) are disturbed over 2-fold, and the most possible biofunctions of myricetin are the effect on "cardiovascular disease, metabolic disease, and lipid metabolism," via regulation of 28 molecules with statistic score of 46. RT-qPCR data confirmed the accuracy of microarray data, and cytokines assay results indicated that 6 of the total 27 inflammatory cytokine secretions were significantly inhibited by myricetin pretreatment, including TNF-α, IFN-γ, IL-1α, IL-1β, IL-2, and IL-6. The present study is the first time to elucidate the multi-function of myricetin in genome-wide set by IPA analysis and verify its anti-inflammatory property by proteomics of cytokines assay. Therefore, these results enrich the comprehensive bioactivities of myricetin and reveal that myricetin has powerful anti-inflammatory property, which provides encouragement for in vivo studies to verify its possible health benefits.

PMID:
30956980
PMCID:
PMC6431437
DOI:
10.1155/2019/3746326
[Indexed for MEDLINE]
Free PMC Article

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