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Vet Microbiol. 2019 Apr;231:191-196. doi: 10.1016/j.vetmic.2019.03.016. Epub 2019 Mar 15.

Genotyping Mycoplasma gallisepticum by multilocus sequence typing.

Author information

1
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary. Electronic address: bekokat@gmail.com.
2
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary. Electronic address: zsuzsakreizinger@yahoo.com.
3
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary. Electronic address: sulyok.kinga@gmail.com.
4
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary. Electronic address: kovcsboti@hotmail.com.
5
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary. Electronic address: groznerdenes@gmail.com.
6
Instituto Zooprofilattico Sperimentale delle Venezie, Via San Giacomo 5, Verona 37000, Italy. Electronic address: scatania@izsvenezie.it.
7
Institute of Veterinary Science, University of Liverpool, Leahurst Campus, Neston CH64 7TE, UK. Electronic address: jmb41@liverpool.ac.uk.
8
Department of Avian and Fish Diseases, Kimron Veterinary Institute, POB 12, Beit Dagan 50250, Israel. Electronic address: lysnyansky@gmail.com.
9
Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia. Electronic address: olaoguno@unimelb.edu.au.
10
NAGISZ Ltd, Fő u. 19, Nádudvar 4181, Hungary. Electronic address: czanikbela@freemail.hu.
11
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Elgomhoria st. 63, Damanhour, Elbehira 22511, Egypt. Electronic address: ellakany_hany@hotmail.com.
12
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária körút 21, Budapest 1143, Hungary; Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Hungária körút 23-25, Budapest 1143, Hungary. Electronic address: m.gyuranecz@gmail.com.

Abstract

Mycoplasma gallisepticum causes chronic respiratory disease and reproductive disorders in many bird species, resulting in considerable economic losses to the poultry industry. Maintenance of M. gallisepticum-free flocks is the most adequate method to control infection. To this end, monitoring systems and vaccination programs with live vaccine strains are applied worldwide. There is strong demand for efficient epidemiological investigation tools to distinguish M. gallisepticum strains in order to control disease. Up to now, multilocus sequence typing (MLST) has been regarded as gold standard for genotyping bacteria due to its good reproducibility and high discriminatory power. The aim of this study was to develop an MLST assay which can determine phylogenetic distances between M. gallisepticum strains. After analysing more than 30 housekeeping genes, six loci (atpG, dnaA, fusA, rpoB, ruvB, uvrA) were selected for the MLST assay due to their genomic location and high diversity. Examination of 130 M. gallisepticum strains with this MLST method yielded 57 unique sequence types (STs) with a 0.96 Simpson's index of diversity. Considering the large number of STs and high diversity index, this MLST method was found to be appropriate to discriminate M. gallisepticum strains. In addition, the developed method was shown to be suitable for epidemiological investigations, as it confirmed linkage between related strains from outbreaks in different farms. Besides, MLST also suggested high impact of extensive international trade on the spread of different M. gallisepticum strains. Furthermore this method can be used for differentiation among vaccine and field strains.

KEYWORDS:

DIVA; Epidemiology; Genotyping; MLST; Mycoplasm gallisepticum

PMID:
30955809
DOI:
10.1016/j.vetmic.2019.03.016
[Indexed for MEDLINE]

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