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Biochimie. 2019 Jul;162:229-238. doi: 10.1016/j.biochi.2019.04.001. Epub 2019 Apr 4.

The apoptosis and GLP-1 hyposecretion induced by LPS via RIP/ROS/mTOR pathway in GLUTag cells.

Author information

1
Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China.
2
Department of Thoracic Oncology, Henan Provincial Chest Hospital, 450008, Zhengzhou, China.
3
Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China. Electronic address: wangshoujun02@126.com.
4
Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China. Electronic address: hyqingj@zzu.edu.cn.

Abstract

Lipopolysaccharide (LPS) as a component of the outer structure of cell wall of gram-negative bacteria, could induce apoptosis in the intestinal endocrine cell line STC-1. However, the signaling cascades involved in this process have not been elucidated. Hence, we investigated the mechanism of cell apoptosis and hyposecretion of glucagon-like peptide 1 (GLP-1) induced by LPS in the GLUTag enteroendocrine cell line. LPS decreased the cell viability of GLUTag cells, up-regulated the TNF-α level, induced the apoptosis and down-regulated the mRNA and protein levels of GLP-1. In addition, TNF-α promoted LPS-induced apoptosis of GLUTag cells through mediating the formation of the RIP1/RIP3 necrosome. RIP1 and RIP3 knockdown increased cell viability, the mRNA and protein levels of GLP-1 and the mTOR signaling pathway-related proteins (p-mTOR and p-S6), and decreased the relative caspase 3/7 activity, cell apoptosis and ROS production. Further studies showed that ROS inhibited the mTOR signaling pathway. Moreover, the antioxidant N-acetyl-l-cysteine increased cell viability, GLP-1 expressions and the mTOR signaling pathway-related proteins, and inhibited the ROS production. However, the mTOR specific inhibitor (Rapa) reversed all these above effects. Taken together, our result revealed that LPS induced the apoptosis of GLUTag cells and GLP-1 hyposecretion through the RIP/ROS/mTOR pathway.

KEYWORDS:

Glucagon-like peptide 1; Lipopolysaccharide; Murine L cell apoptosis; RIP/ROS pathway; mTOR pathway

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