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Biochem Biophys Res Commun. 2019 May 21;513(1):213-218. doi: 10.1016/j.bbrc.2019.03.208. Epub 2019 Apr 4.

NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L.

Author information

1
Department of Bioenergy Science and Technology, Chonnam National University, Gwangju, 61186, South Korea.
2
Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan, 58545, South Korea.
3
Department of Biotechnology, Chonnam National University, Gwangju, 61186, South Korea.
4
Department of Ecosystem Science and Management, Department of Plant Science, Pennsylvania State University, University Park, PA, 16802, USA.
5
Department of Bioenergy Science and Technology, Chonnam National University, Gwangju, 61186, South Korea. Electronic address: asjsuse@jnu.ac.kr.

Abstract

Rare cold-inducible 2 (RCI2) proteins are small hydrophobic proteins that are known to be localized in cellular membranes. The function of RCI2 proteins has been reported to be associated with low-temperature, salt, and drought stress tolerances as a membrane potential regulator; however, the specific functions are still unknown. The PIP2 (plasma membrane intrinsic protein 2) aquaporins are proteins that transport water and small solutes into the cell. The expression and activity of PIP2 proteins, like RCI2, are also related to salt- and drought-stress tolerance. In this study, we identified novel protein interactions between RCI2 and PIP2; 1, including protein accumulation changes in the bioenergy crop Camelina sativa L. under various NaCl stress conditions. Accumulation of both CsRCI2E and CsRCI2F proteins increased with NaCl stress; however, to differing levels depending on the NaCl stress intensity. A co-immunoprecipitation test revealed interaction between CsRCI2E-CsPIP2 and CsRCI2F-CsPIP2. Moreover, co-expression of the four CsRCI2 proteins with CsPIP2; 1 in Xenopus laevis oocytes reduced water transport activity. Furthermore, the abundance of CsPIP2; 1 protein was decreased under CsRCI2E and CsRCI2F co-expression. These results suggest that NaCl-induced expression of CsRCI2E and CsRCI2F contributes to the regulation of CsPIP2; 1.

KEYWORDS:

Camelina sativa L.; PIP2; Plasma membrane; Protein interaction; RCI2s; Salt stress

PMID:
30954220
DOI:
10.1016/j.bbrc.2019.03.208

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