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Blood Cells Mol Dis. 2019 Jul;77:34-42. doi: 10.1016/j.bcmd.2019.03.009. Epub 2019 Mar 30.

Increased CD8+CD27+perforin+ T cells and decreased CD8+CD70+ T cells may be immune biomarkers for aplastic anemia severity.

Author information

1
Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China.
2
Department of Hematology, Guangzhou First Municipal People's Hospital, The Second Affiliated Hospital of South China University of Technology, Guangzhou, China.
3
Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China.
4
Guangzhou Blood Center, Guangzhou, China.
5
Department of Cardiology, First Affiliated Hospital, Jinan University, Guangzhou, China.
6
Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, China.
7
Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China; Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China; Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, China. Electronic address: yangqiuli@hotmail.com.
8
Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China; Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China. Electronic address: libo517@jnu.edu.cn.

Abstract

OBJECTIVES:

Aplastic anemia (AA) is T cell immune-mediated autoimmune disease. Aberrant T cell activation involves an imbalance in T cell homeostasis in AA. However, whether the T cell activation molecule CD27 and its ligand CD70 participate in the immune pathogenesis of AA remains ill defined.

METHODS:

The frequencies of CD27/CD70 and perforin/granzyme B in different T cell subsets were detected in AA patients and healthy individuals by flow cytometry.

RESULTS:

We first time demonstrate a significantly elevated proportion of CD27+ and significantly decreased CD70+ T cells from AA. Changed frequency of CD27+ and CD70+ in different T cell subsets appeared to be associated with AA severity. In very severe aplastic anemia (VSAA) and severe aplastic anemia (SAA), increased CD8+CD27+ T cells present with a cytotoxic effector phenotype by elevating perforin proportion.

CONCLUSIONS:

Elevated proportion of CD27 in T cells may contribute to distinct immune pathogenesis for different severities of AA. The CD8+CD27+perforin+ T cells combined with CD8+CD70+ T cells may serve as an immune biomarker for AA severity estimation.

KEYWORDS:

Aplastic anemia; CD27; CD70; Perforin

PMID:
30953940
DOI:
10.1016/j.bcmd.2019.03.009

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