Format

Send to

Choose Destination
Aesthet Surg J. 2019 Apr 6. pii: sjz099. doi: 10.1093/asj/sjz099. [Epub ahead of print]

Deposition of Host Matrix Proteins on Breast Implant Surfaces Facilitates Staphylococcus Epidermidis Biofilm Formation: In Vitro Analysis.

Author information

1
Washington University School of Medicine, St. Louis, MO.

Abstract

BACKGROUND:

Staphylococcus epidermidis is a primary cause of breast implant-associated infection. S. epidermidis possesses several virulence factors that enable it to bind both abiotic surfaces and host factors to form a biofilm. S. epidermidis also colocalizes with matrix proteins coating explanted human breast implants.

OBJECTIVES:

To identify matrix proteins that S. epidermidis may exploit to infect various breast implant surfaces in vitro.

METHODS:

A combination of in vitro assays was used to characterize S. epidermidis strains isolated from human breast implants to gain a better understanding of how these bacteria colonize breast implant surfaces. These included determining the: (1) minimum inhibitory and bactericidal concentrations for irrigation solutions commonly used to prevent breast implant contamination; (2) expression and carriage of polysaccharide intercellular adhesin and serine-aspartate repeat proteins, which bind fibrinogen (SdrG) and collagen (SdrF), respectively; and (3) biofilm formation on varying implant surface characteristics, in different growth media, and supplemented with fibrinogen and Types I and III collagen. Scanning electron microscopy and immunofluorescence staining analyses were performed to corroborate findings from these assays.

RESULTS:

Textured breast implant surfaces support greater bacterial biofilm formation at baseline, while the addition of collagen significantly increases biomass on all surfaces tested. We found that S. epidermidis isolated from breast implants all encoded SdrF. Consistent with this finding, these strains had a clear affinity for Type I collagen, forming dense, highly structured biofilms in its presence.

CONCLUSIONS:

S . epidermidis may utilize SdrF to interact with Type I collagen to form biofilm on breast implant surfaces.

PMID:
30953053
DOI:
10.1093/asj/sjz099

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center