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Anticancer Res. 2019 Apr;39(4):1807-1812. doi: 10.21873/anticanres.13287.

Preliminary Observation on the Cytotoxic Activity of New Chlorophyllin Derivative RCD on Human Tumour Cell Lines In Vitro.

Author information

1
Iraklis Galatoulas Research Unit, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy arnaldo.pinelli@unimi.it.
2
Iraklis Galatoulas Research Unit, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy.

Abstract

BACKGROUND:

Chlorophyllin is used in traditional Chinese medicine because of its anticancer properties. This article describes the preparation and cytotoxic activity of a reduced chlorophyllin derivative (RCD) on tumour cell lines.

MATERIALS AND METHODS:

RCD was prepared by reducing chlorophyllin with lithium aluminium hydride, and its solubility in the alkaline and organic phases are different from that of the parent compound chlorophyllin.

RESULTS:

RCD also has different chromatographic behaviour from chlorophyllin on thin-layer chromatography and high-pressure liquid chromatography, and excitation and emission spectra. RCD has cytotoxic activity against ZR-75, MCF-7 and HT-29, human tumour cell lines. A clonogenic assay showed that the growth of tumour colonies on soft agar was reduced by RCD in a dose-dependent manner.

CONCLUSION:

RCD is a novel compound exhibiting anticancer activity.

KEYWORDS:

Chlorophyllin; RCD; cytotoxic activity; reduced chlorophyllin derivative; reduction; tumour cell lines

PMID:
30952720
DOI:
10.21873/anticanres.13287
[Indexed for MEDLINE]

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