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Tissue Eng Part A. 2019 Apr 5. doi: 10.1089/ten.TEA.2018.0342. [Epub ahead of print]

A Biomimicking Polymeric Cryogel Scaffold for Repair of Critical Sized Cranial Defect in Rat Model.

Liu C1,2, Lin C3,4, Feng X3,5, Wu Z6, Lin G7, Quan C8, Chen B9, Zhang C10.

Author information

1
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instruments, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China.
2
School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China ; liuchunt@mail.sysu.edu.cn.
3
Department of Orthopedics & Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
4
Department of Orthopaedics and Traumatology, The First People's Hospital of FoShan (Affiliated FoShan Hospital of Sun Yet-sen University), Foshan, Guangdong, China ; lincw1021@sina.com.
5
Department of Orthopaedics and Traumatology, Queen Mary Hospital, the University of Hong Kong, Hong Kong, China ; fengxiaoreng@hku.hk.
6
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instruments, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, Guangdong, China ; wuzhy37@mail.sysu.edu.cn.
7
Department of Orthopedics & Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China ; 1609968669@qq.com.
8
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instruments, School of Biomedical Engineering, Sun Yat-sen University,, Guangzhou, Guangdong, China ; quancy2010@163.com.
9
Department of Orthopedics & Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China ; chb@smu.edu.cn.
10
School of Biomedical Engineering , Rm B517 Engineering Building , Sun Yat-Sen University , 132 East Wauhuan Road, Guangzhou Higher Education Mega Centre , Guangzhou, Guangdong, China , 510006 ; zhchao9@mail.sysu.edu.cn.

Abstract

Mineralized polymeric cryogels with interconnective macroporous structure have demonstrated their potential as promising scaffolding material in bone tissue engineering. However, their capability in inducing osteogenic differentiation of MSCs in vitro and osteogenesis in vivo have not been explored yet. In this work, the roles of the mineralized cryogel on osteogenesis are systematically studied. Mineralized macroporous poly(ethylene glycol)-co-2-hydroxyethyl methacrylate cryogel promotes osteogenic differentiation of rat mesenchymal stem cells (rMSCs), particularly in upregulating the activity of alkaline phosphatase (ALP, ~5.7 folds) and expression of related osteogenic gene markers (ALP ~16 folds, osteocalcin (OCN) ~133 folds) at 14 days. In vivo implantation reveals that mineralized cryogels could promote fast osteogenesis and angiogenesis in critical-sized cranial bone defect of a Sprague Dawley rat model in 4 weeks. The adsorption, entrapment, and concentration of osteogenic growth factors (bone morphogenetic protein 2, or BMP2) and angiogenesis growth factor (vascular endothelial growth factor, or VEGF) in the matrices in vivo may possibly participate in the process of osteogenesis and angiogenesis. Notably, the adsorption of larger amount of VEGF in non-mineralized cryogels facilitate obvious angiogenesis and comparable osteogenesis in bone defect in 8 weeks.

PMID:
30950322
DOI:
10.1089/ten.TEA.2018.0342

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