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Biomed Microdevices. 2019 Apr 4;21(2):39. doi: 10.1007/s10544-019-0397-6.

Investigation of parameters that determine Nano-DC vaccine transport.

Author information

1
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, 77030, USA.
2
Xiangya Hospital of Central South University, Changsha, 410000, Hunan, China.
3
Center for Bioenergetics, Houston Methodist Research Institute, Houston, TX, 77030, USA.
4
State key laboratory of ophthalmology, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
5
Center for Inflammation and Epigenetics, Houston Methodist Research Institute, Houston, TX, 77030, USA.
6
Department of Surgery, Brigham and Women's Hospital, Dana-Farber/ Brigham and Women's Cancer Center, Boston, MA, 02115, USA.
7
Breast Oncology Program, Dana-Farber/ Brigham and Women's Cancer Center, Boston, MA, 02115, USA.
8
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, 77030, USA. mferrari@houstonmethodist.org.
9
Department of Medicine, Weill Cornell Medical College, 1300 York Avenue, New York, NY, 10065, USA. mferrari@houstonmethodist.org.
10
Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, 77030, USA. hshen@houstonmethodist.org.
11
Department of Cell and Developmental Biology, Weill Cornell Medical College, 1300 York Avenue, New York, NY, 10065, USA. hshen@houstonmethodist.org.

Abstract

Effective migration of dendritic cells into the lymphatic system organs is the prerequisite for a functional dendritic cell vaccine. We have previously developed a porous silicon microparticle (PSM)-based therapeutic dendritic cell vaccine (Nano-DC vaccine) where PSM serves both as the vehicle for antigen peptides and an adjuvant. Here, we analyzed parameters that determined dendritic cell uptake of PSM particles and Nano-DC vaccine accumulation in lymphatic tissues in a murine model of HER2-positive breast cancer. Our study revealed a positive correlation between sphericity of the PSM particles and their cellular uptake by circulating dendritic cells. In addition, the intravenously administered vaccines accumulated more in the spleens and inguinal lymph nodes, while the intradermally inoculated vaccines got enriched in the popliteal lymph nodes. Furthermore, mice with large tumors received more vaccines in the lymph nodes than those with small to medium size tumors. Information from this study will provide guidance on design and optimization of future therapeutic cancer vaccines.

KEYWORDS:

Biodistribution; Microparticle; Nano-DC vaccine; Silicon; Transport

PMID:
30949852
DOI:
10.1007/s10544-019-0397-6

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