Send to

Choose Destination
Science. 2019 Apr 5;364(6435):52-56. doi: 10.1126/science.aav6428.

Defining the developmental program leading to meiosis in maize.

Author information

Department of Biology, Stanford University, Stanford, CA 94305, USA.


In multicellular organisms, the entry into meiosis is a complex process characterized by increasing meiotic specialization. Using single-cell RNA sequencing, we reconstructed the developmental program into maize male meiosis. A smooth continuum of expression stages before meiosis was followed by a two-step transcriptome reorganization in leptotene, during which 26.7% of transcripts changed in abundance by twofold or more. Analysis of cell-cycle gene expression indicated that nearly all pregerminal cells proliferate, eliminating a stem-cell model to generate meiotic cells. Mutants defective in somatic differentiation or meiotic commitment expressed transcripts normally present in early meiosis after a delay; thus, the germinal transcriptional program is cell autonomous and can proceed despite meiotic failure.


Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center