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Science. 2019 Apr 5;364(6435):52-56. doi: 10.1126/science.aav6428.

Defining the developmental program leading to meiosis in maize.

Author information

1
Department of Biology, Stanford University, Stanford, CA 94305, USA. bnelms.research@gmail.com walbot@stanford.edu.

Abstract

In multicellular organisms, the entry into meiosis is a complex process characterized by increasing meiotic specialization. Using single-cell RNA sequencing, we reconstructed the developmental program into maize male meiosis. A smooth continuum of expression stages before meiosis was followed by a two-step transcriptome reorganization in leptotene, during which 26.7% of transcripts changed in abundance by twofold or more. Analysis of cell-cycle gene expression indicated that nearly all pregerminal cells proliferate, eliminating a stem-cell model to generate meiotic cells. Mutants defective in somatic differentiation or meiotic commitment expressed transcripts normally present in early meiosis after a delay; thus, the germinal transcriptional program is cell autonomous and can proceed despite meiotic failure.

PMID:
30948545
DOI:
10.1126/science.aav6428

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