Format

Send to

Choose Destination
Tissue Cell. 2019 Apr;57:49-56. doi: 10.1016/j.tice.2019.02.002. Epub 2019 Feb 14.

In vivo articular cartilage regeneration through infrapatellar adipose tissue derived stem cell in nanofiber polycaprolactone scaffold.

Author information

1
Department of Anatomical Sciences, Faculty of Medicine, Maragheh University of Medical Sciences, Maragheh, Iran. Electronic address: pa.vahedi48@gmail.com.
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
3
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
4
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran" and "Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran".

Abstract

In this study, we report the development of a nanofiber polycaprolactone scaffold that can act as a stem cell carrier to induce chondrogenesis and promote cartilage repair in vivo. Infrapatellar fat pads were obtained from sheep knee and the stem cells were isolated and characterized by flow cytometry. Defects were created in sheep knee, two defects received adipose tissue derived stem cells (ASCs)-polycaprolactone construct, second group received polycaprolactone (PCL), the third group was chosen as the ASCs group and the fourth group was control group. Morphological evaluation showed that defects treated with ASCs-scaffold constructs were completely filled with cartilage-like tissue, while other groups revealed the formation of a thin layer of cartilage-like tissue in the defects. Real-Time RT-PCR showed the increase in collagen type 2 mRNA levels, aggrecan and Sox9 in ASCs/PCL groups in comparison with the other groups. Immunofluorescence and toluidine blue staining results showed the protein expression of collagen type 2 and formation of round and polygonal clusters of chondrocytes in ASCS/PCL group. According to our results nanofiber polycaprolactone promoted the chondrogenesis of infrapatellar adipose tissue derived stem cells in vivo and could offer significant promise in the biological functionality of stem cell tissue engineering in clinical practice.

KEYWORDS:

Cell therapy; Chondrogenesis; Hyaline cartilage; Osteoarthritis; Polycaprolactone; Stem cell transplantation

PMID:
30947963
DOI:
10.1016/j.tice.2019.02.002

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center