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PLoS One. 2019 Apr 4;14(4):e0203977. doi: 10.1371/journal.pone.0203977. eCollection 2019.

Quality assessment of tissue samples stored in a specialized human lung biobank.

Author information

1
Asklepios Biobank for Lung Diseases, Department of Thoracic Surgery, Asklepios Fachkliniken München-Gauting, Gauting and German Center for Lung Research - DZL, Munich, Germany.
2
German Center for Lung Research - DZL, Munich, Germany.
3
Asklepios Biobank for Lung Diseases, Department of Pathology, Asklepios Fachkliniken München-Gauting, Gauting, Germany.
4
Institute of Medical Informatics, Statistics and Epidemiology, Klinikum rechts der Isar, Technical University Munich, Munich, Germany.

Abstract

Human sample, from patients or healthy donors, are a valuable link between basic research and clinic. Especially in translational research, they play an essential role in understanding development and progression of diseases as well as in developing new diagnostic and therapeutic tools. Stored in biobanks, fast access to appropriate material becomes possible. However, biobanking in a clinical context faces several challenges. In practice, collecting samples during clinical routine does not allow to strictly adhere to protocols of sample collection in all aspects. This may influence sample quality to variable degrees. Time from sample draw to asservation is a variable factor, and influences of prolonged storage at ambient temperature of tissues are not well understood. We investigated whether delays between 5 minutes and 3 hours, and the use of RNAlater RNA-preserving reagent would lead to a relevant drop in sample quality, measured by quantitative mRNA expression analysis. Our findings suggest that even under ambient conditions, delays up to 3 hours do not have a major impact on sample quality as long as the tissue remains intact.

Conflict of interest statement

The authors have declared that no competing interests exist.

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