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Methods Mol Biol. 2019;1933:389-394. doi: 10.1007/978-1-4939-9045-0_24.

Transcriptome-Wide Mapping 5-Methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant.

Author information

1
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China. guxiaofeng@caas.cn.
2
Department of Biological Sciences, National University of Singapore, Singapore, Singapore.

Abstract

Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m5C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m5C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.

KEYWORDS:

5-Methylcytosine; Plant; RNA immunoprecipitation; RNA methylation; m5C; m5C-RIP-seq

PMID:
30945199
DOI:
10.1007/978-1-4939-9045-0_24
[Indexed for MEDLINE]

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