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J BUON. 2019 Jan-Feb;24(1):99-105.

High expression of Plnc RNA-1 promotes the progression of colorectal cancer.

Author information

1
Department of General Surgery, The Chengyang People's Hospital, Qingdao, China.

Abstract

PURPOSE:

To study the expression of Plnc RNA-1 in colorectal cancer tissues and cells, and to explore the role of Plnc RNA-1 in the regulation of cell cycle and in the progression of colorectal cancer.

METHODS:

A total of 77 cases of colorectal cancer tissues were retrospectively analyzed. Thirty colorectal normal tissues composed the control group. The expression of Plnc RNA-1 in colorectal cancer tissues at different stages was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) so as to analyze the influence of Plnc RNA-1 on the survival of colorectal cancer patients and the accuracy of colorectal cancer diagnosis. Besides, SW116 and LOVO cell lines were transfected with si-Plnc RNA-1, pc-DNA-Plnc RNA-1 as well as their negative controls to achieve Plnc RNA-1 knockdown or overexpression. Then, we detected the transfection efficiency by qRT-PCR. Furthermore, cell counting kit-8 (CCK-8) and colony formation assays were performed to explore the effect of Plnc RNA-1 on colorectal cancer cells proliferation. Flow cytometry was used to examine the effect of Plnc RNA-1 on the cell cycle. In addition, western blotting was used to detect the expression levels of p-GSK3β, p-Rb and CyclinD1.

RESULTS:

The expression level of Plnc RNA-1 in 77 colorectal cancer tissues was significantly higher than that of the control. Plnc RNA-1 expression level in patients with infiltrating T3+T4 stages was higher than that in infiltrating T1+T2 stages. In stages III+IV patients, the expression level of Plnc RNA-1 was higher than that of stages I+II. Plnc RNA-1 high expression group exhibited significantly lower survival rate than Plnc RNA-1 low expression group, suggesting there was a significant positive correlation between the sensitivity of colorectal cancer diagnosis and the expression of Plnc RNA-1. Overexpression of Plnc RNA-1 could significantly increase the viability and proliferation in the SW116 cells. In the LOVO cell line, knockdown of Plnc RNA-1 significantly decreased cell viability and proliferation. In the LOVO cell line, knockdown of Plnc RNA-1 promoted cell cycle and decreased the expression of cell cycle-related proteins p-GSK3β, p-Rb and cyclinD1.In the SW116 cell line, overexpression of Plnc RNA-1 led to cell cycle arrest and increased the expression of cell cycle-related proteins.

CONCLUSIONS:

The expression of Plnc RNA-1 in colorectal cancer cells was significantly upregulated. Plnc RNA-1 participated in the development of colorectal cancer through regulating the cell cycle, which may provide a new theoretical basis for the treatment of colorectal cancer and a new therapeutic target.

PMID:
30941957

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