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J BUON. 2019 Jan-Feb;24(1):61-67.

Effect of miR-200c on the proliferation, migration and invasion of breast cancer cells and relevant mechanisms.

Author information

1
Department of Endocrinology and Metabolism, the Second Hospital of Changzhou Affiliated to Nanjing Medical University, Changzhou, Jiangsu 213003, China.

Abstract

PURPOSE:

The current study aimed to explore the effect of miR-200c on the proliferation, migration and invasion of breast cancer cells and its relevant mechanisms.

METHODS:

Cell counting kit-8 (CCK-8), scratch wound healing assay and Transwell assay were performed after upregulation of miR-200c to detect the capabilities of proliferation, migration and invasion of MCF-7 breast cancer cells. Also, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were carried out to determine the expression levels of fucosyltransferase-4 (FUT4) and relevant genes in PI3K/AKT signaling pathways.

RESULTS:

miR-200c upregulation in MCF-7 cells decreased the capabilities of proliferation, migration and invasion in MCF-7 cells. MiR-200c could regulate the level of FUT4 in MCF-7 cells, and might affect the cell proliferation, migration and invasion through PI3K/AKT signaling pathway.

CONCLUSIONS:

The results of this study indicated that miR-200c might serve as a new target in the diagnosis and treatment of breast cancer. MiR-200c regulated the expression of FUT4, and affected the biological behaviors of breast cancer MCF-7 cells, such as proliferation, migration and invasion.

PMID:
30941952

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