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Nat Commun. 2019 Apr 2;10(1):1500. doi: 10.1038/s41467-019-09488-0.

Cortex-wide neural interfacing via transparent polymer skulls.

Author information

1
Department of Mechanical Engineering, University of Minnesota, Twin Cities, MN, USA.
2
Department of Neuroscience, University of Minnesota, Twin Cities, MN, USA.
3
Department of Biomedical Engineering, University of Minnesota, Twin Cities, MN, USA.
4
Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA.
5
Department of Mechanical Engineering, University of Minnesota, Twin Cities, MN, USA. suhasabk@umn.edu.
6
Department of Biomedical Engineering, University of Minnesota, Twin Cities, MN, USA. suhasabk@umn.edu.

Abstract

Neural computations occurring simultaneously in multiple cerebral cortical regions are critical for mediating behaviors. Progress has been made in understanding how neural activity in specific cortical regions contributes to behavior. However, there is a lack of tools that allow simultaneous monitoring and perturbing neural activity from multiple cortical regions. We engineered 'See-Shells'-digitally designed, morphologically realistic, transparent polymer skulls that allow long-term (>300 days) optical access to 45 mm2 of the dorsal cerebral cortex in the mouse. We demonstrate the ability to perform mesoscopic imaging, as well as cellular and subcellular resolution two-photon imaging of neural structures up to 600 µm deep. See-Shells allow calcium imaging from multiple, non-contiguous regions across the cortex. Perforated See-Shells enable introducing penetrating neural probes to perturb or record neural activity simultaneously with whole cortex imaging. See-Shells are constructed using common desktop fabrication tools, providing a powerful tool for investigating brain structure and function.

PMID:
30940809
PMCID:
PMC6445105
DOI:
10.1038/s41467-019-09488-0
[Indexed for MEDLINE]
Free PMC Article

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