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Biomaterials. 2019 Jun;206:160-169. doi: 10.1016/j.biomaterials.2019.03.036. Epub 2019 Mar 27.

A 3D cell printed muscle construct with tissue-derived bioink for the treatment of volumetric muscle loss.

Author information

1
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea; Materials Processing Innovation Research Division, Department of Advanced Biomaterials Research, Korea Institute of Materials Science (KIMS). 797 Changwon-daero, Seongsan-gu, Changwon-si, Gyeongsangnam-do, 51508, South Korea.
2
Department of Plastic Surgery, The Catholic University of Korea, 222 Banpodaero, Seocho-gu, Seoul 06591, South Korea.
3
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea.
4
Department of Computer Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea.
5
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea.
6
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea; Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea.
7
Department of Plastic Surgery, The Catholic University of Korea, 222 Banpodaero, Seocho-gu, Seoul 06591, South Korea. Electronic address: rhie@catholic.ac.kr.
8
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea. Electronic address: dwcho@postech.ac.kr.

Abstract

Volumetric muscle loss (VML) is an irrecoverable injury associated with muscle loss greater than 20%. Although hydrogel-based 3D engineered muscles and the decellularized extracellular matrix (dECM) have been considered for VML treatment, they have shown limited efficacy. We established a novel VML treatment with dECM bioink using 3D cell printing technology. Volumetric muscle constructs composed of cell-laden dECM bioinks were generated with a granule-based printing reservoir. The 3D cell printed muscle constructs exhibited high cell viability without generating hypoxia and enhanced de novo muscle formation in a VML rat model. To improve functional recovery, prevascularized muscle constructs that mimic the hierarchical architecture of vascularized muscles were fabricated through coaxial nozzle printing with muscle and vascular dECM bioinks. Spatially printing tissue-specific dECM bioinks offers organized microenvironmental cues for the differentiation of each cell and improves vascularization, innervation, and functional recovery. Our present results suggest that a 3D cell printing and tissue-derived bioink-based approach could effectively generate biomimetic engineered muscles to improve the treatment of VML injuries.

KEYWORDS:

3D bioprinting; 3D cell printing; Bioink; Decellularized extracellular matrix; Tissue engineering; Volumetric muscle loss

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