Format

Send to

Choose Destination
ACS Appl Mater Interfaces. 2019 Apr 24;11(16):14608-14618. doi: 10.1021/acsami.9b01532. Epub 2019 Apr 15.

Sustained Release SDF-1α/TGF-β1-Loaded Silk Fibroin-Porous Gelatin Scaffold Promotes Cartilage Repair.

Author information

1
Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital , Jinan University , Guangzhou 510630 , P. R. China.
2
Department of Genetics , University of Cambridge , Cambridge CB2 3EH , United Kingdom.
3
Department of Histology and Embryology, Joint Laboratory for Embryonic Development & Prenatal Medicine, Medical College , Jinan University , Guangzhou 510632 , Guangdong , P. R. China.

Abstract

Continuous delivery of growth factors to the injury site is crucial to creating a favorable microenvironment for cartilage injury repair. In the present study, we fabricated a novel sustained-release scaffold, stromal-derived factor-1α (SDF-1α)/transforming growth factor-β1 (TGF-β1)-loaded silk fibroin-porous gelatin scaffold (GSTS). GSTS persistently releases SDF-1α and TGF-β1, which enhance cartilage repair by facilitating cell homing and chondrogenic differentiation. Scanning electron microscopy showed that GSTS is a porous microstructure and the protein release assay demonstrated the sustainable release of SDF-1α and TGF-β1 from GSTS. Bone marrow-derived mesenchymal stem cells (MSCs) maintain high in vitro cell activity and excellent cell distribution and phenotype after seeding into GSTS. Furthermore, MSCs acquired enhanced chondrogenic differentiation capability in the TGF-β1-loaded scaffolds (GSTS or GST: loading TGF-β1 only) and the conditioned medium from SDF-1α-loaded scaffolds (GSTS or GSS: loading SDF-1α only) effectively promoted MSCs migration. GSTS was transplanted into the osteochondral defects in the knee joint of rats, and it could promote cartilage regeneration and repair the cartilage defects at 12 weeks after transplantation. Our study shows that GSTS can facilitate in vitro MSCs homing, migration, chondrogenic differentiation and SDF-1α and TGF-β1 have a synergistic effect on the promotion of in vivo cartilage forming. This SDF-1α and TGF-β1 releasing GSTS have promising therapeutic potential in cartilage repair.

KEYWORDS:

SDF-1α; TGF-β1; cartilage; gelatin; silk fibroin

PMID:
30938503
DOI:
10.1021/acsami.9b01532

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center