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Dev Dyn. 2019 Apr 2. doi: 10.1002/dvdy.32. [Epub ahead of print]

Stable transgenesis in Astyanax mexicanus using the Tol2 transposase system.

Author information

1
Department of Biological Sciences, Florida Atlantic University, Florida.
2
Jupiter Life Science Initiative, Florida Atlantic University, Florida.
3
Stowers Institute for Medical Research, Kansas City, Missouri.
4
Department of Ecology, Evolution, and Behavior, University of Minnesota, St. Paul, Minnesota.
5
Wilkes Honors College, Florida Atlantic University, Jupiter, Florida.
6
Department of Molecular and Integrative Physiology, KU Medical Center, Kansas City, Kansas.

Abstract

BACKGROUND:

Astyanax mexicanus is a well-established fish model system for evolutionary and developmental biology research. These fish exist as surface forms that inhabit rivers and 30 different populations of cavefish. Despite important progress in the deployment of new technologies, deep mechanistic insights into the genetic basis of evolution, development, and behavior have been limited by a lack of transgenic lines commonly used in genetic model systems.

RESULTS:

Here, we expand the toolkit of transgenesis by characterizing two novel stable transgenic lines that were generated using the highly efficient Tol2 system, commonly used to generate transgenic zebrafish. A stable transgenic line consisting of the zebrafish ubiquitin promoter expresses enhanced green fluorescent protein ubiquitously throughout development in a surface population of Astyanax. To define specific cell-types, a Cntnap2-mCherry construct labels lateral line mechanosensory neurons in zebrafish. Strikingly, both constructs appear to label the predicted cell types, suggesting many genetic tools and defined promoter regions in zebrafish are directly transferrable to cavefish.

CONCLUSION:

The lines provide proof-of-principle for the application of Tol2 transgenic technology in A. mexicanus. Expansion on these initial transgenic lines will provide a platform to address broadly important problems in the quest to bridge the genotype-phenotype gap.

PMID:
30938001
DOI:
10.1002/dvdy.32

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