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ACS Cent Sci. 2019 Mar 27;5(3):515-523. doi: 10.1021/acscentsci.8b00916. Epub 2019 Feb 11.

One- and Two-Dimensional High-Resolution NMR from Flat Surfaces.

Author information

1
Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
2
Department of Chemistry and Applied Biosciences, ETH Zürich, Vladimir Prelog Weg 1-5, CH-8093 Zürich, Switzerland.
3
Department of Chemistry, Iowa State University, Ames, Iowa 50011-3020, United States.
4
Institut de Sciences Analytiques, Centre de RMN à Très Hauts Champs, Université de Lyon (CNRS/ENS Lyon/UCB Lyon 1), 69100 Villeurbanne, France.

Abstract

Determining atomic-level characteristics of molecules on two-dimensional surfaces is one of the fundamental challenges in chemistry. High-resolution nuclear magnetic resonance (NMR) could deliver rich structural information, but its application to two-dimensional materials has been prevented by intrinsically low sensitivity. Here we obtain high-resolution one- and two-dimensional 31P NMR spectra from as little as 160 picomoles of oligonucleotide functionalities deposited onto silicate glass and sapphire wafers. This is enabled by a factor >105 improvement in sensitivity compared to typical NMR approaches from combining dynamic nuclear polarization methods, multiple-echo acquisition, and optimized sample formulation. We demonstrate that, with this ultrahigh NMR sensitivity, 31P NMR can be used to observe DNA bound to miRNA, to sense conformational changes due to ion binding, and to follow photochemical degradation reactions.

Conflict of interest statement

The authors declare no competing financial interest.

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