Format

Send to

Choose Destination
J Neurosci. 2019 Jun 12;39(24):4797-4813. doi: 10.1523/JNEUROSCI.0839-18.2019. Epub 2019 Apr 1.

Modulators of Kv3 Potassium Channels Rescue the Auditory Function of Fragile X Mice.

Author information

1
Departments of Pharmacology.
2
Surgery.
3
Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P. R. China.
4
Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, P.R. China, and.
5
Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai 200125, P. R. China.
6
Autifony Therapeutics Limited, Stevenage Bioscience Catalyst, Stevenage SG1 2FX, United Kingdom.
7
Neuroscience.
8
Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520.
9
Departments of Pharmacology, leonard.kaczmarek@yale.edu.

Abstract

Fragile X syndrome (FXS) is characterized by hypersensitivity to sensory stimuli, including environmental sounds. We compared the auditory brainstem response (ABR) recorded in vivo in mice lacking the gene (Fmr1 -/y ) for fragile X mental retardation protein (FMRP) with that in wild-type animals. We found that ABR wave I, which represents input from the auditory nerve, is reduced in Fmr1 -/y animals, but only at high sound levels. In contrast, wave IV, which represents the activity of auditory brainstem nuclei is enhanced at all sound levels, suggesting that loss of FMRP alters the central processing of auditory signals. Current-clamp recordings of neurons in the medial nucleus of the trapezoid body in the auditory brainstem revealed that, in contrast to neurons from wild-type animals, sustained depolarization triggers repetitive firing rather than a single action potential. In voltage-clamp recordings, K+ currents that activate at positive potentials ("high-threshold" K+ currents), which are required for high-frequency firing and are carried primarily by Kv3.1 channels, are elevated in Fmr1 -/y mice, while K+ currents that activate near the resting potential and inhibit repetitive firing are reduced. We therefore tested the effects of AUT2 [((4-({5-[(4R)-4-ethyl-2,5-dioxo-1-imidazolidinyl]-2-pyridinyl}oxy)-2-(1-methylethyl) benzonitrile], a compound that modulates Kv3.1 channels. AUT2 reduced the high-threshold K+ current and increased the low-threshold K+ currents in neurons from Fmr1 -/y animals by shifting the activation of the high-threshold current to more negative potentials. This reduced the firing rate and, in vivo, restored wave IV of the ABR. Our results from animals of both sexes suggest that the modulation of the Kv3.1 channel may have potential for the treatment of sensory hypersensitivity in patients with FXS.SIGNIFICANCE STATEMENT mRNA encoding the Kv3.1 potassium channel was one of the first described targets of the fragile X mental retardation protein (FMRP). Fragile X syndrome is caused by loss of FMRP and, in humans and mice, causes hypersensitivity to auditory stimuli. We found that components of the auditory brain response (ABR) corresponding to auditory brainstem activity are enhanced in mice lacking FMRP. This is accompanied by hyperexcitability and altered potassium currents in auditory brainstem neurons. Treatment with a drug that alters the voltage dependence of Kv3.1 channels normalizes the imbalance of potassium currents, as well as ABR responses in vivo, suggesting that such compounds may be effective in treating some symptoms of fragile X syndrome.

KEYWORDS:

AUT2; auditory brainstem response; fragile X; high- and low-threshold potassium channels; medial nucleus of the trapezoid body; potassium channels

PMID:
30936239
PMCID:
PMC6561694
[Available on 2019-12-12]
DOI:
10.1523/JNEUROSCI.0839-18.2019

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center