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Int J Biol Macromol. 2019 Jul 1;132:844-851. doi: 10.1016/j.ijbiomac.2019.03.210. Epub 2019 Mar 29.

Characterization of Moringa oleifera roots polysaccharide MRP-1 with anti-inflammatory effect.

Author information

1
Department of Pharmaceutical Engineering, College of Materials and Energy, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, South China Agricultural University, Guangzhou 510642, PR China.
2
Department of Immunology and Infectious Diseases, John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
3
Department of Pharmaceutical Engineering, College of Materials and Energy, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangdong Province Research Center of Woody Forage Engineering Technology, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: wanglei@scau.edu.cn.

Abstract

Moringa oleifera is a mutli-purpose herbal plant which has attained enormous attention as a natural source of nutrients and folk medicine. This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column. Monosaccharide composition analysis based on GC-MS showed that MRP-1 mainly consisted of Rha, Ara, Fru, Xyl, Man and Gal in the molar ratio of 1.5:2.0:3.1:6.0:5.3:1.1. The Roman spectra, FT-IR and NMR analysis showed that the typical features of carbohydrates, such as α-Araf, α-Gly, β-Galp, α-GalpA and β-Gly was contained by MRP-1. The LPS-induced RAW264.7 macrophage cells were used to evaluate the anti-inflammatory activity of MRP-1. The result demonstrated that the increasing of NO and TNF-α production induced by LPS could be prevented by different concentrations of MRP-1 treatment. Moreover, the mRNA expression level of iNOS induced by LPS was decreased significantly (p < 0.05) by MRP-1 treatment while show no obvious effect on the COX-2 mRNA expression. This study may provide new possible application of Moringa oleifera root polysaccharide related to anti-inflammation.

KEYWORDS:

Anti-inflammatory activity, TNF-α; Moringa oleifera root; Polysaccharides purification; iNOS mRNA

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