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Angew Chem Int Ed Engl. 2019 May 27;58(22):7395-7399. doi: 10.1002/anie.201902929. Epub 2019 Apr 23.

Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles.

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PASTEUR, Département de Chimie, École Normale Superiéure, PSL University, Sorbonne Université, CNRS, 24 rue Lhomond, 75005, Paris, France.
UMR7099, Institut de Biologie Physico-Chimique, CNRS, Univ. Paris Diderot, Sorbonne Université, 13 rue Pierre et Marie Curie, 75005, Paris, France.
Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
Present Address: Department of Microbiology, Institute for Water and Wetland Research, Heyendaalseweg 135, 6525, Nijmegen, The Netherlands.
PPSM, CNRS, École Normale Supérieure Paris-Saclay, Université Paris-Saclay, 61 Avenue du Président Wilson, 94235, Cachan, France.


Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high-yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria-based proteoliposomes are an attractive eco-friendly alternative that can outperform currently used liposomes.


bacterial production; click chemistry; encapsulation; nanovesicle; proteoliposome


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