Format

Send to

Choose Destination
PLoS One. 2019 Apr 1;14(4):e0210374. doi: 10.1371/journal.pone.0210374. eCollection 2019.

Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.

Author information

1
All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences, Moscow, Russia.
2
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
3
Evrogen JSC, Moscow, Russia.
4
Pirogov Russian National Research Medical University, Moscow, Russia.
5
The Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
6
Syntol JSC, Moscow, Russia.

Abstract

Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.

PMID:
30933980
PMCID:
PMC6443234
DOI:
10.1371/journal.pone.0210374
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

K.A. Blagodatskikh is employed by Evrogen JSC. Y.A. Monakhova is employed by Syntol JSC. T.V. Kramarova is currently employed by Anocca AB. SD polymerase used in this project is the subject of a patent application (US20160145588, EP2981609). This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center