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Nat Prod Res. 2019 Mar 31:1-6. doi: 10.1080/14786419.2019.1586699. [Epub ahead of print]

Bioactivity-based analysis and chemical characterization of cytotoxic compounds from a poisonous mushroom, Amanita spissacea, in human lung cancer cells in vitro.

Author information

1
a School of Pharmacy , Sungkyunkwan University , Suwon , Republic of Korea.
2
b Department of Molecular and Cellular Biology, Samsung Medical Center , Sungkyunkwan University School of Medicine , Suwon , Republic of Korea.
3
c Agricultural Microbiology Division , National Institute of Agricultural Sciences, RDA , Wanju-gun , Jeollabuk-do , Republic of Korea.
4
d Special Forest Products Division, Forest Bioresources Department , National Institute of Forest Science , Suwon , Republic of Korea.

Abstract

As part of our systematic study on Korean toxic mushrooms, bioactivity-guided fractionation of the MeOH extract of Amanita spissacea (Amanitaceae) fruiting bodies and chemical investigation of its cytotoxic fractions led to the isolation of (9E)-8-oxo-9-octadecenoic acid (1), (10E)-9-oxo-10-octadecenoic acid (2), (9E)-8-oxo-9-octadecenoate methyl ester (3), (9Z)-9-octadecenoate-(2'S)-2',3'-dihydroxypropyl ester (4), (9Z)-9-octadecenoic acid (5), and palmitic acid (6). The structures of the isolates were elucidated by NMR spectroscopic analysis and LC/MS analysis. Among the isolated compounds, compounds 1 and 2 exhibited the most potent cytotoxic activity in all human lung cancer cell lines examined, with IC50 values ranging from 255.7 to 321.0 μM and 250.2 to 322.5 μM, respectively. The cytotoxicity of these compounds was also found to be mediated by apoptosis associated with caspase-3 activation. These findings provide experimental evidence suggesting the potential of A. spissacea as a promising natural source for the discovery of novel anticancer drug candidates.

KEYWORDS:

; apoptosis; cytotoxicity; fatty acids; lung cancer; poisonous mushroom

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