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J Mater Sci Mater Med. 2019 Mar 30;30(4):43. doi: 10.1007/s10856-019-6241-y.

Osteoblast adhesion and response mediated by terminal -SH group charge surface of SiOxCy nanowires.

Author information

1
Centro Universitario di Odontoiatria, University of Parma, Via Gramsci 14, 43126, Parma, Italy.
2
Dipartimento di Medicina e Chirurgia, University of Parma, Via Gramsci 14, 43126, Parma, Italy.
3
ISMAC-CNR, Institute for macromolecular studies, Via Corti, 12, 20133, Milano, Italy. lagonegro@ismac.cnr.it.
4
IMEM-CNR, Institute of Materials for Electronics and Magnetism, Parco Area delle Scienze, 37/A, 43124, Parma, Italy. lagonegro@ismac.cnr.it.
5
School of Medicine, University of Genoa, DIMES, L.go R. Benzi 10, Genoa, 16131, Italy.
6
IMEM-CNR, Institute of Materials for Electronics and Magnetism, Trento unit, Via alla Cascata, 56/C, 38123, Trento, Italy.
7
IMEM-CNR, Institute of Materials for Electronics and Magnetism, Parco Area delle Scienze, 37/A, 43124, Parma, Italy.
8
ISMAC-CNR, Institute for macromolecular studies, Via Corti, 12, 20133, Milano, Italy.

Abstract

Robust cell adhesion is known to be necessary to promote cell colonization of biomaterials and differentiation of progenitors. In this paper, we propose the functionalization of Silicon Oxycarbide (SiOxCy) nanowires (NWs) with 3-mercaptopropyltrimethoxysilane (MPTMS), a molecule containing a terminal -SH group. The aim of this functionalization was to develop a surface capable to adsorb proteins and promote cell adhesion, proliferation and a better deposition of extracellular matrix. This functionalization can be used to anchor other structures such as nanoparticles, proteins or aptamers. It was observed that surface functionalization markedly affected the pattern of protein adsorption, as well as the in vitro proliferation of murine osteoblastic cells MC3T3-E1, which was increased on functionalized nanowires (MPTMS-NWs) compared to bare NWs (control) (pā€‰<ā€‰0.0001) after 48ā€‰h. The cells showed a better adhesion on MPTMS-NWs than on bare NWs, as confirmed by immunofluorescence studies on the cytoskeleton, which showed a more homogeneous vinculin distribution. Gene expression analysis showed higher expression levels for alkaline phosphatase and collagen I, putative markers of the osteoblast initial differentiation stage. These results suggest that functionalization of SiOxCy nanowires with MPTMS enhances cell growth and the expression of an osteoblastic phenotype, providing a promising strategy to improve the biocompatibility of SiOxCy nanowires for biomedical applications.

PMID:
30929122
DOI:
10.1007/s10856-019-6241-y

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