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Plant Physiol Biochem. 2019 Jun;139:314-324. doi: 10.1016/j.plaphy.2019.03.033. Epub 2019 Mar 26.

Transcriptome analysis of harvested bell peppers (Capsicum annuum L.) in response to cold stress.

Author information

1
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: 254291390@qq.com.
2
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: 309504752@qq.com.
3
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: 403880675@qq.com.
4
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: bdweisyau@163.com.
5
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: 1300570891@qq.com.
6
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: 958826636@qq.com.
7
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: yb_zhao1979@163.com.
8
Department of Food Science, Shenyang Agricultural University, Shenyang, 110866, PR China. Electronic address: jsjsyau@yeah.net.

Abstract

Bell peppers are valued for their plentiful vitamin C and nutritional content. Pepper fruits are susceptible to cold storage, which leads to chilling injury (CI); however, the crucial metabolic product and molecular basis response to cold stress have not been elucidated definitely yet. To comprehensively understand the gene regulation network and CI mechanisms in response to cold stress on a molecular level, we performed high-throughput RNA-Seq analysis to investigate genome-wide expression profiles in bell peppers at different storage temperatures (4 °C and 10 °C). A total of 61.55 Gb of clean data were produced; 3863 differentially expressed genes (DEGs) including 1669 up-regulated and 2194 down-regulated were annotated and classified between the CI group and control. Together, a total of 41 cold-induced transcription factor families comprising 250 transcription factors (TFs) were identified. Notably, numerous DEGs involved in biomembrane stability, dehydration and osmoregulation, and plant hormone signal transduction processes were discovered. The transcriptional level of 20 DEGs was verified by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Our results present transcriptome profiles of bell peppers in response to cold stress; the data obtained may be useful for the identification of key candidate genes and elucidation of the mechanisms underlying membrane damage during chilling injury.

KEYWORDS:

Bell peppers; Chilling injury; Differentially expressed genes; Gene annotation; RNA-Seq; RT-qPCR

PMID:
30927694
DOI:
10.1016/j.plaphy.2019.03.033
[Indexed for MEDLINE]

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