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Nanoscale Res Lett. 2019 Mar 28;14(1):109. doi: 10.1186/s11671-019-2941-y.

Tailoring Cell Morphomechanical Perturbations Through Metal Oxide Nanoparticles.

Author information

1
Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento, Via Arnesano, 73100, Lecce, Italy. valeria.dematteis@unisalento.it.
2
Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento, Via Arnesano, 73100, Lecce, Italy.
3
Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.
4
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, 08028, Barcelona, Spain.

Abstract

The nowadays growing use of nanoparticles (NPs) in commercial products does not match a comprehensive understanding of their potential harmfulness. More in vitro investigations are required to address how the physicochemical properties of NPs guide their engulfment within cells and their intracellular trafficking, fate, and toxicity. These nano-bio interactions have not been extensively addressed yet, especially from a mechanical viewpoint. Cell mechanic is a critical indicator of cell health because it regulates processes like cell migration, tissue integrity, and differentiation via cytoskeleton rearrangements. Here, we investigated in vitro the elasticity perturbation of Caco-2 and A549 cell lines, in terms of Young's modulus modification induced by SiO2NPS and TiO2NPS. TiO2NPs demonstrated stronger effects on cell elasticity compared to SiO2NPs, as they induced significant morphological and morphometric changes in actin network. TiO2NPS increased the elasticity in Caco-2 cells, while opposite effects have been observed on A549 cells. These results demonstrate the existence of a correlation between the alteration of cell elasticity and NPs toxicity that depends, in turn, on the NPs physicochemical properties and the specific cell tested.

KEYWORDS:

Biomechanics; Cytoskeleton rearrangements; Nanoparticles; Toxicity; Young’s Modulus

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