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Sci Rep. 2019 Mar 28;9(1):5291. doi: 10.1038/s41598-019-41832-8.

RNA interference-mediated knockdown of voltage-gated sodium channel (MpNav) gene causes mortality in peach-potato aphid, Myzus persicae.

Author information

1
Department of Agriculture, Abdul Wail Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan. drkaleem@awkum.edu.pk.
2
Department of Agriculture, Abdul Wail Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan.
3
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
4
Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.
5
State Key Laboratory of Agricultural Microbiology; Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
6
Department of Entomology, College of Plant protection, China Agriculture University, Beijing, 100193, China.

Abstract

Voltage-gated sodium channels (VGSC) are transmembrane proteins that generate an action potential in excitable cells and play an essential role in neuronal signaling. Since VGSCs play a crucial role in nerve transmission they have become primary targets for a broad range of commercial insecticides. RNA interference (RNAi) is a valuable reverse genetics tool used in functional genomics, but recently, it has also shown promise as a novel agent that could be used to control agricultural insect pests. In this study, we targeted the VGSC (MpNav) gene in the peach-potato aphid Myzus persicae, by oral feeding of artificial diets mixed with dsRNAs. Knock-down of MpNav gene expression caused up to 65% mortality in 3rd instar nymphs. Moreover, significantly lower fecundity and longevity was observed in adult aphids that had been fed with dsMpNav solution at the nymphal stage. Analysis of gene expression by qRT-PCR indicated that the aphid mortality rates and the lowered fecundity and longevity were attributable to the down-regulation of MpNav by RNAi. Taken together, our results show that MpNav is a viable candidate target gene for the development of an RNAi-based bio-aphicide.

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