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Science. 2019 Mar 29;363(6434):1456-1459. doi: 10.1126/science.aaw0046.

Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth.

Author information

1
Laboratory of Plant Physiology and Biophysics, Institute of Molecular, Cell and Systems Biology, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK.
2
Plant Science Group, Institute of Molecular, Cell and Systems Biology, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK.
3
Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
4
Plant Science Group, Institute of Molecular, Cell and Systems Biology, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK. michael.blatt@glasgow.ac.uk john.christie@glasgow.ac.uk.
5
Laboratory of Plant Physiology and Biophysics, Institute of Molecular, Cell and Systems Biology, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK. michael.blatt@glasgow.ac.uk john.christie@glasgow.ac.uk.
#
Contributed equally

Abstract

Stomata serve dual and often conflicting roles, facilitating carbon dioxide influx into the plant leaf for photosynthesis and restricting water efflux via transpiration. Strategies for reducing transpiration without incurring a cost for photosynthesis must circumvent this inherent coupling of carbon dioxide and water vapor diffusion. We expressed the synthetic, light-gated K+ channel BLINK1 in guard cells surrounding stomatal pores in Arabidopsis to enhance the solute fluxes that drive stomatal aperture. BLINK1 introduced a K+ conductance and accelerated both stomatal opening under light exposure and closing after irradiation. Integrated over the growth period, BLINK1 drove a 2.2-fold increase in biomass in fluctuating light without cost in water use by the plant. Thus, we demonstrate the potential of enhancing stomatal kinetics to improve water use efficiency without penalty in carbon fixation.

PMID:
30923223
DOI:
10.1126/science.aaw0046

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