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FASEB J. 2019 Mar 28:fj201802536RR. doi: 10.1096/fj.201802536RR. [Epub ahead of print]

Small ribonucleoprotein particle protein SmD3 governs the homeostasis of germline stem cells and the crosstalk between the spliceosome and ribosome signals in Drosophila.

Author information

1
Department of Gynecology, The Affiliated Hospital of Jiangsu University-Jiangsu University, Zhenjiang, China.
2
Reproductive Sciences Institute, Jiangsu University, Zhenjiang, China.
3
Department of Clinical Pharmacy, The Affiliated Hospital of Jiangsu University-Jiangsu University, Zhenjiang, China.
4
Center for Reproduction and Genetics, Suzhou Municipal Hospital-The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China.
5
Center for Reproduction, The Fourth Affiliated Hospital of Jiangsu University-The Fourth People's Hospital of Zhenjiang, Zhenjiang, China.
6
Department of Obstetrics and Gynecology, The Fourth Affiliated Hospital of Jiangsu University-The Fourth People's Hospital of Zhenjiang, Zhenjiang, China.
7
Department of Clinical Medicine, China Medical University, Shenyang, China.
8
Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
9
Department of Urology, The Affiliated Hospital of Jiangsu University.

Abstract

The ribonucleoprotein (RNP) spliceosome machinery triggers the precursor RNA splicing process in eukaryotes. Major spliceosome defects are implicated in male infertility; however, the underlying mechanistic links between the spliceosome and the ribosome in Drosophila testes remains largely unresolved. Small ribonucleoprotein particle protein SmD3 (SmD3) is a novel germline stem cell (GSC) regulatory gene identified in our previous screen of Drosophila testes. In the present study, using genetic manipulation in a Drosophila model, we demonstrated that SmD3 is required for the GSC niche and controls the self-renewal and differentiation of GSCs in the testis. Using in vitro assays in Schneider 2 cells, we showed that SmD3 also regulates the homeostasis of proliferation and apoptosis in Drosophila. Furthermore, using liquid chromatography-tandem mass spectrometry methods, SmD3 was identified as binding with ribosomal protein (Rp)L18, which is a key regulator of the large subunit in the ribosome. Moreover, SmD3 was observed to regulate spliceosome and ribosome subunit expression levels and controlled spliceosome and ribosome function via RpL18. Significantly, our findings revealed the genetic causes and molecular mechanisms underlying the stem cell niche and the crosstalk between the spliceosome and the ribosome.-Yu, J., Luan, X., Yan, Y., Qiao, C., Liu, Y., Zhao, D., Xie, B., Zheng, Q., Wang, M., Chen, W., Shen, C., He, Z., Hu, X., Huang, X., Li, H., Chen, B., Zheng, B., Chen, X., Fang, J. Small ribonucleoprotein particle protein SmD3 governs the homeostasis of germline stem cells and the crosstalk between the spliceosome and ribosome signals in Drosophila.

KEYWORDS:

GSCs; RpL18; SmD3

PMID:
30921522
DOI:
10.1096/fj.201802536RR

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