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Bioelectromagnetics. 2019 Apr;40(3):180-187. doi: 10.1002/bem.22181.

Involvement of calcium in 50-Hz magnetic field-induced activation of sphingosine kinase 1 signaling pathway.

Author information

1
Bioelectromagnetics Key Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
2
Department of Occupational Disease of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
3
Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Abstract

Previously, we found that exposure to a 50-Hz magnetic field (MF) could induce human amniotic epithelial (FL) cell proliferation and sphingosine kinase 1 (SK1) activation, but the mechanism was not clearly understood. In the present study, the possible signaling pathways which were involved in SK1 activation induced by 50-Hz MF exposure were investigated. Results showed that MF exposure increased intracellular Ca2+ which was dependent on the L-type calcium channel, and induced Ca2+ -dependent phosphorylation of extracellular regulated protein kinase (ERK), SK1, and protein kinase C α (PKCα). Also, treatment with U0126, an inhibitor of ERK, could block MF-induced SK1 phosphorylation, but had no effect on PKCα phosphorylation. Also, the inhibitor of PKCα, Gö6976, had no effect on MF-induced SK1 activation in FL cells. In addition, the activation of ERK and PKCα could be abolished by SKI II, the inhibitor of SK1. In conclusion, the intracellular Ca2+ mediated the 50-Hz MF-induced SK1 activation which enhanced PKCα phosphorylation, and there might be a feedback mechanism between SK1 and ERK activation in responding to MF exposure in FL cells. Bioelectromagnetics. 9999:XX-XX, 2019.

KEYWORDS:

50-Hz magnetic field (MF); Sphingosine kinase 1 (SK1); extracellular regulated protein kinase (ERK); intracellular Ca2+; protein kinase Cα (PKCα)

PMID:
30920672
DOI:
10.1002/bem.22181

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