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J Asthma. 2019 Mar 28:1-9. doi: 10.1080/02770903.2018.1563902. [Epub ahead of print]

Validation of Strasbourg environmental exposure chamber (EEC) ALYATEC® in mite allergic subjects with asthma.

Author information

a Chest Diseases Department , Strasbourg University Hospital , Strasbourg , France.
b Federation of Translational Medicine EA 3070 , University of Strasbourg , Strasbourg , France.
c Clinics of Immuno-Allergology, CHU Brugmann , Université Libre de Bruxelles (ULB) , Brussels , Belgium.
d Biocluster des Haras , ALYATEC® , Strasbourg , France.
e François-Rabelais University , Tours Cedex , France.



Environmental Exposure Chamber (EEC) should have standardized and controlled allergenic and non-allergenic exposures to perform reproducible clinical studies. The aim was to demonstrate that mite exposure in the Alyatec® EEC could induce early (EAR) and/or late asthmatic reactions (LAR) in at least 60% of subjects allergic to mite.


The EEC has a volume of 147-m3 with 20 seats. The nebulized particle number, airborne Der p1, endotoxins, and volatile organic compound (VOC) concentrations were measured. Twenty-four asthmatics allergic to mite were randomly exposed to 15, 25, and 46 ng/m3 Der p1. Specificity was assessed in not mite-sensitized asthmatics.


No significant endotoxin or VOC contamination was measured. The mean inter-assay CVs were 12.5% for the airborne particle number and 28.7% for airborne Der p1 concentrations. For the three Der p1 concentrations, at least 88% of the subjects developed EAR and/or LAR, and at least 46% developed a dual response. No reaction occurred with placebo or in the control group. No severe bronchial reaction occurred.


The Alyatec® EEC demonstrated a tight control of allergenic and non-allergenic exposures. The EEC was clinically validated, with airborne Der p1 levels close to levels found in natural settings.


Mite; asthma; early asthmatic response; environmental experimental chamber; late asthmatic response

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