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Arch Gynecol Obstet. 2019 Jun;299(6):1701-1707. doi: 10.1007/s00404-019-05111-1. Epub 2019 Mar 27.

The myo-inositol effect on the oocyte quality and fertilization rate among women with polycystic ovary syndrome undergoing assisted reproductive technology cycles: a randomized clinical trial.

Author information

1
Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences, PO Box: 15875-1454, 1168743514, Tehran, Iran.
2
Department of Anatomy, School of Medicine Sciences, Iran University of Medical Sciences, Tehran, Iran.
3
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
4
Basir Eye Health Research Center, Tehran, Iran.
5
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
6
Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences, PO Box: 15875-1454, 1168743514, Tehran, Iran. Amjadi.fs@iums.ac.ir.
7
Department of Anatomy, School of Medicine Sciences, Iran University of Medical Sciences, Tehran, Iran. Amjadi.fs@iums.ac.ir.

Abstract

PURPOSE:

The aim of the present study was to evaluate the effect of myo-Inositol administration on oocyte quality, fertilization rate and embryo quality in patients with PCOS during assisted reproductive technology (ART) cycles.

METHODS:

Fifty infertile PCOS patients were randomly designated in two groups. In the study group, patients received daily doses of 4 g myo-Inositol combined with 400 mg folic acid and in the control group patients received only 400 mg folic acid from 1 month before starting the antagonist cycle until the day of ovum pick up. Oocyte and embryo qualities were assessed according to European Society of Human Reproduction and Embryology (ESHRE) guidelines. The gene expression of PGK1, RGS2 and CDC42 as a factor of oocyte quality in granulosa cells was analyzed using real-time RT-PCR. Levels of total antioxidant capacity (TAC) and reactive oxygen species (ROS) were evaluated by chemiluminescence assay in follicular fluid.

RESULTS:

The percentage of metaphase II oocyte, fertilization rate and embryo quality significantly improved in the study group (p < 0.05), but the number of retrieved oocytes and follicle count were not statistically different between groups. Furthermore, the gene expression of PGK1, RGS2 and CDC42 was significantly higher in the study group (p < 0.05) but no differences were found between two groups in terms of TAC and ROS levels.

CONCLUSIONS:

The present study findings suggest that myo-Inositol alters the gene expression in granulosa cells and improves oocyte and embryo quality among PCOS patients undergoing ART.

KEYWORDS:

Assisted reproductive technology; Fertilization; Oocyte; Polycystic ovary syndrome; myo-Inositol

PMID:
30919036
DOI:
10.1007/s00404-019-05111-1

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