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Elife. 2019 Mar 26;8. pii: e43882. doi: 10.7554/eLife.43882.

Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury.

Farbehi N#1,2,3,4, Patrick R#1,2,5, Dorison A1,2, Xaymardan M1,2,6, Janbandhu V1,2,5, Wystub-Lis K1, Ho JW1,5, Nordon RE2,4, Harvey RP1,2,7.

Author information

Victor Chang Cardiac Research Institute, Darlinghurst, Australia.
Stem Cells Australia, Melbourne Brain Centre, University of Melbourne, Victoria, Australia.
Garvan Weizmann Centre for Cellular Genomics, Garvan Institute of Medical Research, Sydney, Australia.
Graduate School of Biomedical Engineering, UNSW Sydney, Kensington, Australia.
St. Vincent's Clinical School, UNSW Sydney, Kensington, Australia.
School of Dentistry, Faculty of Medicine and Health, University of Sydney, Westmead Hospital, Westmead, Australia.
School of Biotechnology and Biomolecular Science, UNSW Sydney, Kensington, Australia.
Contributed equally


Besides cardiomyocytes (CM), the heart contains numerous interstitial cell types which play key roles in heart repair, regeneration and disease, including fibroblast, vascular and immune cells. However, a comprehensive understanding of this interactive cell community is lacking. We performed single-cell RNA-sequencing of the total non-CM fraction and enriched (Pdgfra-GFP+) fibroblast lineage cells from murine hearts at days 3 and 7 post-sham or myocardial infarction (MI) surgery. Clustering of >30,000 single cells identified >30 populations representing nine cell lineages, including a previously undescribed fibroblast lineage trajectory present in both sham and MI hearts leading to a uniquely activated cell state defined in part by a strong anti-WNT transcriptome signature. We also uncovered novel myofibroblast subtypes expressing either pro-fibrotic or anti-fibrotic signatures. Our data highlight non-linear dynamics in myeloid and fibroblast lineages after cardiac injury, and provide an entry point for deeper analysis of cardiac homeostasis, inflammation, fibrosis, repair and regeneration.


cell biology; computational biology; heart; mouse; myocardial infarction; scRNA-seq; systems biology

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