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Molecules. 2019 Mar 22;24(6). pii: E1138. doi: 10.3390/molecules24061138.

Use of 13C-qNMR Spectroscopy for the Analysis of Non-Psychoactive Cannabinoids in Fibre-Type Cannabis sativa L. (Hemp).

Author information

1
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy. lucia.marchetti@unimore.it.
2
Doctorate School in Clinical and Experimental Medicine (CEM), University of Modena and Reggio Emilia, 41125 Modena, Italy. lucia.marchetti@unimore.it.
3
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy. virginia.brighenti@unimore.it.
4
Centro Interdipartimentale Grandi Strumenti, University of Modena and Reggio Emilia, Via G. Campi 213/A, 41125 Modena, Italy. mariacecilia.rossi@unimore.it.
5
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy. johanna.sperlea@ernaehrung.uni-giessen.de.
6
Faculty of Agricultural Sciences, Nutritional Sciences, and Environmental Management, Justus-Liebig University of Giessen, Goethestrasse 58, 35390 Giessen, Germany. johanna.sperlea@ernaehrung.uni-giessen.de.
7
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy. federica.pellati@unimore.it.
8
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy. davide.bertelli@unimore.it.

Abstract

Cannabis sativa L. is a dioecious plant belonging to the Cannabaceae family. The discovery of the presence of many biologically-active metabolites (cannabinoids) in fibre-type Cannabis (hemp) has recently given rise to the valorisation of this variety. In this context, the present study was aimed at the multi-component analysis and determination of the main non-psychoactive cannabinoids (cannabidiol, cannabidiolic acid, cannabigerol and cannabigerolic acid) in female inflorescences of different hemp varieties by means of 13C quantitative nuclear magnetic resonance spectroscopy (qNMR). The method proposed here for the first time for the determination of cannabinoids provided reliable results in a competitive time with respect to the more consolidated HPLC technique. In fact, it gave sufficiently precise and sensitive results, with LOQ values lower than 750 μg/mL, which is easily achievable with concentrated extracts, without affecting the quality of 13C-qNMR spectra. In conclusion, this method can be considered as a promising and appropriate tool for the comprehensive chemical analysis of bioactive cannabinoids in hemp and other derived products in order to ensure their quality, efficacy and safety.

KEYWORDS:

13C-qNMR; Cannabis sativa L.; HPLC; cannabidiol; cannabigerol; cannabinoids; hemp

PMID:
30909372
PMCID:
PMC6470610
DOI:
10.3390/molecules24061138
[Indexed for MEDLINE]
Free PMC Article

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