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ACS Nano. 2019 Apr 23;13(4):4312-4321. doi: 10.1021/acsnano.8b09519. Epub 2019 Mar 27.

N- and B-Codoped Graphene: A Strong Candidate To Replace Natural Peroxidase in Sensitive and Selective Bioassays.

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Department of Chemical Engineering , Pohang University of Science and Technology (POSTECH) , Pohang , Gyeongbuk 37673 , Republic of Korea.
Department of Chemical and Biomolecular Engineering , Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141 , Republic of Korea.
Department of BioNano Technology , Gachon University , Seongnam , Gyeonggi 13120 , Republic of Korea.
Department of Energy Engineering, School of Energy and Chemical Engineering , Ulsan National Institute of Science and Technology (UNIST) , Ulsan 44919 , Republic of Korea.


The work describes a carbon-based peroxidase mimic, N- and B-codoped reduced graphene oxide (NB-rGO), which shows high peroxidase-like activity without oxidase-like activity and has a catalytic efficiency nearly 1000-fold higher than that of undoped rGO. The high catalytic activity of NB-rGO is explained by density functional theory by calculating Gibbs free energy change during the peroxide decomposition reaction. Acetylcholine and C-reactive protein are successfully quantified with high sensitivity and selectivity, which were comparable to or better than those obtained using natural peroxidase. Furthermore, NB-rGO, which does not have oxidase-like activity, is proven to have higher sensitivity toward acetylcholine than Pt nanoparticles having oxidase-like activity. This work will facilitate studies on development, theoretical analysis for rational design, and bioassay applications of enzyme mimics based on nanomaterials.


biosensors; density functional theory; enzyme mimic; graphene; immunoassays


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