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J Vis Exp. 2019 Mar 7;(145). doi: 10.3791/58932.

Wet-spinning-based Molding Process of Gelatin for Tissue Regeneration.

Author information

1
Department of Materials Science and Engineering, National Taiwan University of Science and Technology.
2
Laboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center.
3
School of Dentistry, National Yang-Ming University.
4
Laboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center; Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center; Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences.
5
Laboratory of Adult Stem Cell and Tissue Regeneration, National Defense Medical Center; Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center.
6
Department of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center.
7
Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center; christmas1035@hotmail.com.
8
Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center; doc20283@gmail.com.

Abstract

This article presents an inexpensive method to fabricate gelatin, as a natural polymer, into monofilament fibers or other appropriate forms. Through the wet spinning method, gelatin fibers are produced by smooth extrusion in a suitable coagulation medium. To increase the functional surface of these gelatin fibers and their ability to mimic the features of tissues, gelatin can be molded into a tube form by referring to this concept. Examined by in vitro and in vivo tests, the gelatin tubes demonstrate a great potential for application in tissue engineering. Acting as a suitable filling gap material, gelatin tubes can be used to substitute the tissue in the damaged area (e.g., in the nervous or cardiovascular system), as well as to promote regeneration by providing a direct replacement of stem cells and neural circuitry. This protocol provides a detailed procedure for creating a biomaterial based on a natural polymer, and its implementation is expected to greatly benefit the development of correlative natural polymers, which help to realize tissue regeneration strategies.

PMID:
30907875
DOI:
10.3791/58932

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