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Histol Histopathol. 2019 Mar 25:18104. doi: 10.14670/HH-18-104. [Epub ahead of print]

Implications on pathogenesis and risk of oral lichen planus neoplastic transformation: an ex-vivo retrospective immunohistochemical study.

Author information

1
Laboratories of applied Microbiology, Department of Health Sciences (DiSS), Università del Piemonte Orientale (UPO), Novara, Italy.
2
Center of Allergic and Autoimmune Diseases (CAAD), DiSS, UPO, Novara, Italy.
3
Unit of Medical Statistics and Epidemiology, Department of Translational Medicine (DiMet), UPO and CPO-Piemonte, Novara, Italy.
4
biomedical Materials, Department of Health Sciences (DiSS), Università del Piemonte Orientale (UPO), Novara, Italy.
5
Dentistry Unit, Azienda Ospedaliero-Universitaria (AOU) "Maggiore della Carità", DiMeT, UPO, Novara, Italy.
6
Immunology, Department of Health Sciences (DiSS), Università del Piemonte Orientale (UPO), Novara, Italy.
7
Interdisciplinary Research Center of Autoimmune Diseases (IRCAD), DiSS, UPO, Novara, Italy.
8
Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM), Firenze, Local Unit UPO, Novara, Italy.
9
Laboratories of applied Microbiology, Department of Health Sciences (DiSS), Università del Piemonte Orientale (UPO), Novara, Italy. barbara.azzimonti@med.uniupo.it.
10
Pathology Unit, Ospedale "Sant'Andrea", DiMeT, UPO, Vercelli, Italy.
#
Contributed equally

Abstract

AIMS:

to evaluate OPN, MCM7, Ki-67, p53, Bcl-2 and 53BP1 presence, together with the abnormal adaptive CD4 and CD8 T-cell response markers expression in a series of oral lichen planus (OLP) affected patients and assess their combined contribution for a more objective disease classification.

METHODS AND RESULTS:

In this ex-vivo retrospective analysis, biopsy specimens from 28 adults with a clinical diagnosis of OLP at different progression degree (16 reticular, 2 plaque-like, 1 erosive and 9 mixed type) were collected. Sections were immunohistochemically investigated for the proinflammatory cytokine osteopontin (OPN), alpha-beta CD4 and CD8 positive T cells, DNA replication licensing factor (MCM7), proliferating cell marker (Ki-67), apoptotic and tumor antigen (p53), apoptosis modulator (Bcl-2) and cellular response regulator to double-strand breaks tumor suppressor p53-binding protein 1 expression. Statistical analysis revealed that 53BP1 is highly represented among the OLP study patients (p<0.05). Moreover, on the basis of the quantification results of the highly expressed parameters, two illness categories with different severity were evidenced. The classification hypothesis was confirmed by i) OLP lesion persistence, ii) the development of oral severe lesions in the patients belonging to high grade activity OLP group (HGA-OLPs) and iii) the ascertainment of the same evidence both in the oral squamous cell tumor controls (OSCC) and in HGA-OLP cases.

CONCLUSION:

This study completes the scenario with respect to early detection, thanks to a more precise histological analysis, for rationalizing the clinical and histological findings toward a sharable international disease scoring system.

PMID:
30907426
DOI:
10.14670/HH-18-104

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