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Odontology. 2019 Oct;107(4):449-456. doi: 10.1007/s10266-019-00419-5. Epub 2019 Mar 22.

Evaluation of milk fat globule-epidermal growth factor-factor VIII and IL-1β levels in gingival crevicular fluid and saliva in periodontal disease and health.

Author information

1
Department of Periodontology, Faculty of Dentistry, Istanbul Medeniyet University, 34100, Istanbul, Turkey. muscyz111@gmail.com.
2
Oral dental health center, Private practice, Izmir, Turkey.
3
Department of Periodontology, Faculty of Dentistry, Izmir Katip Çelebi University, Izmir, Turkey.
4
Department of Periodontology, Faculty of Dentistry, University of Health Science, Istanbul, Turkey.

Abstract

The aim of this study is to determine the levels of MFG-E8 and interleukin (IL)-1β in saliva and gingival crevicular fluid (GCF) associated with periodontal health and disease. Whole saliva and GCF samples were obtained from systemically healthy participants who were either periodontally healthy (n = 24) or suffered from gingivitis (n = 25) or chronic periodontitis (n = 25). Full-mouth clinical periodontal measurements, including bleeding on probing, probing depth, gingival index, plaque index, and clinical attachment level were also recorded. Enzyme-linked immunosorbent assay was used to estimate MFG-E8 and IL-1β levels in the samples. Analysis of variance, Kruskal-Wallis tests, and Pearson correlation tests were used to analyse the data statistically. The total level of MFG-E8 in GCF was significantly higher in the healthy group than in the other two groups (P = 0.01). Salivary MFG-E8 levels did not differ significantly among the groups. There were negative correlations between the level of MFG-E8 in GCF and probing depth (P = 0.03), bleeding on probing (P = 0.001), plaque index (P = 0.003), and gingival index (P = 0.003). The total level of IL-1β in GCF was significantly lower in the healthy group than in the groups with gingivitis and chronic periodontitis (P < 0.001). Salivary IL-1β levels showed significant differences across all three groups (P < 0.001). The level of MFG-E8 in GCF was higher in the healthy group than in the periodontal disease groups. Furthermore, there was no difference between gingivitis and periodontitis groups. The relationship between MFG-E8 and periodontal status should be further investigated.

KEYWORDS:

Gingival crevicular fluid; IL-1β; MFG-E8; Periodontitis; Saliva

PMID:
30903320
DOI:
10.1007/s10266-019-00419-5

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