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Mol Plant. 2019 Apr 1;12(4):597-602. doi: 10.1016/j.molp.2019.03.006. Epub 2019 Mar 19.

Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding.

Author information

1
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: wangbaobao@caas.cn.
2
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
3
School of Life Sciences, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
4
School of Life Sciences, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China. Electronic address: whyang@scau.edu.cn.

Abstract

Crop breeding aims to generate pure inbred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing technologies in crop breeding. However, both of them still fall short for rapid generation of pure elite lines with integrated favorable traits. Here, we report the development of a Haploid-Inducer Mediated Genome Editing (IMGE) approach, which utilizes a maize haploid inducer line carrying a CRISPR/Cas9 cassette targeting for a desired agronomic trait to pollinate an elite maize inbred line and to generate genome-edited haploids in the elite maize background. Homozygous pure DH lines with the desired trait improvement could be generated within two generations, thus bypassing the lengthy procedure of repeated crossing and backcrossing used in conventional breeding for integrating a desirable trait into elite commercial backgrounds.

KEYWORDS:

CRSIPR/Cas9; crop breeding; doubled haploids (DH); haploid-inducer mediated genome editing (IMGE); maize

PMID:
30902686
DOI:
10.1016/j.molp.2019.03.006
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