Format

Send to

Choose Destination
Int J Mol Sci. 2019 Mar 21;20(6). pii: E1439. doi: 10.3390/ijms20061439.

Protective Effects of Fermented Oyster Extract against RANKL-Induced Osteoclastogenesis through Scavenging ROS Generation in RAW 264.7 Cells.

Author information

1
Freshwater Bioresources Utilization Bureau, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea. jwjeong@nnibr.re.kr.
2
Department of System Management, Korea Lift College, Geochang 50141, Korea. hyunle6869@hanmail.net.
3
National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea. mhhan@mabik.re.kr.
4
Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea. immunkim@jejunu.ac.kr.
5
Department of Molecular Biology, College of Natural Sciences, Dong-eui University, Busan 47340, Korea. parkch@deu.ac.kr.
6
Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, Korea. hongsh@deu.ac.kr.
7
Anti-Aging Research Center, Dong-eui University, Busan 47227, Korea. hongsh@deu.ac.kr.
8
Marine Bioprocess Co. Ltd., Busan 46048, Korea. hansola82@hanmail.net.
9
Department of Oral Pathology and Regenerative Medicine, School of Dentistry, Kyungpook National University, Daegu 41940, Korea. epark@knu.ac.kr.
10
Department of Food Science and Biotechnology, College of Engineering, Kyungsung University, Busan 48434, Korea. theresa10000@naver.com.
11
Department of Biological Sciences, College of Natural Science, Dong-A University, Busan 49315, Korea. shleem@dau.ac.kr.
12
Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea. youjinj@jejunu.ac.kr.
13
Department of Biochemistry, Dong-eui University College of Korean Medicine, Busan 47227, Korea. choiyh@deu.ac.kr.
14
Anti-Aging Research Center, Dong-eui University, Busan 47227, Korea. choiyh@deu.ac.kr.

Abstract

Excessive bone resorption by osteoclasts causes bone loss-related diseases and reactive oxygen species (ROS) act as second messengers in intercellular signaling pathways during osteoclast differentiation. In this study, we explored the protective effects of fermented oyster extract (FO) against receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced osteoclast differentiation in murine monocyte/macrophage RAW 264.7 cells. Our results showed that FO markedly inhibited RANKL-induced activation of tartrate-resistant acid phosphatase and formation of F-actin ring structure. Mechanistically, FO has been shown to down-regulate RANKL-induced expression of osteoclast-specific markers by blocking the nuclear translocation of NF-κB and the transcriptional activation of nuclear factor of activated T cells c1 (NFATc1) and c-Fos. Furthermore, FO markedly diminished ROS production by RANKL stimulation, which was associated with blocking the expression of nicotinamide adenine dinucleotide phosphate oxidase 1 (NOX1) and its regulatory subunit Rac-1. However, a small interfering RNA (siRNA) targeting NOX1 suppressed RANKL-induced expression of osteoclast-specific markers and production of ROS and attenuated osteoclast differentiation as in the FO treatment group. Collectively, our findings suggest that FO has anti-osteoclastogenic potential by inactivating the NF-κB-mediated NFATc1 and c-Fos signaling pathways and inhibiting ROS generation, followed by suppression of osteoclast-specific genes. Although further studies are needed to demonstrate efficacy in in vivo animal models, FO may be used as an effective alternative agent for the prevention and treatment of osteoclastogenic bone diseases.

KEYWORDS:

NF-κB; NOX1; RANKL; ROS; fermented oyster; osteoclast differentiation

PMID:
30901917
DOI:
10.3390/ijms20061439
Free full text

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI)
Loading ...
Support Center