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Sci Rep. 2019 Mar 21;9(1):4996. doi: 10.1038/s41598-019-41149-6.

Gut microbiota profile in children affected by atopic dermatitis and evaluation of intestinal persistence of a probiotic mixture.

Author information

1
Human Microbiome Unit, Bambino Gesù Children's Hospital IRCCS, Rome, Italy.
2
Dermatology Unit, Bambino Gesù Children's Hospital IRCCS, Rome, Italy.
3
Unit of Allergology, Bambino Gesù Children's Hospital IRCCS, Rome, Italy.
4
University Department of Pediatrics, Unit of Immune and Infectious Diseases, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
5
Human Microbiome Unit and Parasitology Unit, Bambino Gesù Children's Hospital IRCCS, Rome, Italy. lorenza.putignani@opbg.net.

Abstract

Atopic dermatitis (AD) has been hypothesised to be associated with gut microbiota (GM) composition. We performed a comparative study of the GM profile of 19 AD children and 18 healthy individuals aimed at identifying bacterial biomarkers associated with the disease. The effect of probiotic intake (Bifidobacterium breve plus Lactobacillus salivarius) on the modulation of GM and the probiotic persistence in the GM were also evaluated. Faecal samples were analysed by real-time PCR and 16S rRNA targeted metagenomics. Although the probiotics, chosen for this study, did not shape the entire GM profile, we observed the ability of these species to pass through the gastrointestinal tract and to persist (only B. breve) in the GM. Moreover, the GM of patients compared to CTRLs showed a dysbiotic status characterised by an increase of Faecalibacterium, Oscillospira, Bacteroides, Parabacteroides and Sutterella and a reduction of short-chain fatty acid (SCFA)-producing bacteria (i.e., Bifidobacterium, Blautia, Coprococcus, Eubacterium and Propionibacterium). Taken togheter these results show an alteration in AD microbiota composition with the depletion or absence of some species, opening the way to future probiotic intervention studies.

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