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Nano Lett. 2019 Apr 10;19(4):2334-2342. doi: 10.1021/acs.nanolett.8b05005. Epub 2019 Mar 29.

Real-Time Detection of Circulating Tumor Cells in Living Animals Using Functionalized Large Gold Nanorods.

Author information

1
Department of Structural Biology , Stanford University , Stanford , California 94305 , United States.
2
Molecular Imaging Program and the Bio-X Program , Stanford University , Stanford , California 94305 , United States.
3
Electrical Engineering , Stanford University , Stanford , California 94305 , United States.
4
Biophysics Program , Stanford University , Stanford , California 94305 , United States.
5
Department of Surgery , Stanford University School of Medicine , Stanford , California 94305 , United States.
6
Department of Medicine , Stanford University School of Medicine , Stanford , California 94305 , United States.
7
The Chan Zuckerberg Biohub , San Francisco , California 94158 , United States.

Abstract

Optical coherence tomography (OCT) can be utilized with significant speckle reduction techniques and highly scattering contrast agents for non-invasive, contrast-enhanced imaging of living tissues at the cellular scale. The advantages of reduced speckle noise and improved targeted contrast can be harnessed to track objects as small as 2 μm in vivo, which enables applications for cell tracking and quantification in living subjects. Here we demonstrate the use of large gold nanorods as contrast agents for detecting individual micron-sized polystyrene beads and single myeloma cells in blood circulation using speckle-modulating OCT. This report marks the first time that OCT has been used to detect individual cells within blood in vivo. This technical capability unlocks exciting opportunities for dynamic detection and quantification of tumor cells circulating in living subjects.

KEYWORDS:

Optical coherence tomography; circulating tumor cells; gold nanorods

PMID:
30895796
DOI:
10.1021/acs.nanolett.8b05005
[Indexed for MEDLINE]

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