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Cell Rep. 2019 Mar 19;26(12):3257-3271.e8. doi: 10.1016/j.celrep.2019.02.065.

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Author information

1
Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France; Aix-Marseille University, CNRS, INSERM, CIML, Marseille, France.
2
Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France.
3
Aix-Marseille University, CNRS, INSERM, CIML, Marseille, France.
4
ICEP Platform, Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France; MI-Mabs, Aix-Marseille University, Marseille, France.
5
Sorbonne Université, Institut de Biology Paris-Seine, Laboratoire de Biologie du Développement, Paris, France.
6
Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France; Aix-Marseille University, CNRS, Centrale Marseille, I2M, Marseille, France.
7
Department of Biology, University of York, York YO10 5DD, UK.
8
Kennedy Institute of Rheumatology, University of Oxford, Oxford, OX3 7FY, UK.
9
Department of Bio-Pathology, Institut Paoli-Calmettes, Aix-Marseille University, Marseille, France.
10
Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France. Electronic address: michel.aurrand-lions@inserm.fr.
11
Aix-Marseille University, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France; Aix-Marseille University, CNRS, INSERM, CIML, Marseille, France. Electronic address: stephane.mancini@inserm.fr.

Abstract

In the bone marrow, CXCL12 and IL-7 are essential for B cell differentiation, whereas hematopoietic stem cell (HSC) maintenance requires SCF and CXCL12. Peri-sinusoidal stromal (PSS) cells are the main source of IL-7, but their characterization as a pro-B cell niche remains limited. Here, we characterize pro-B cell supporting stromal cells and decipher the interaction network allowing pro-B cell retention. Preferential contacts are found between pro-B cells and PSS cells, which homogeneously express HSC and B cell niche genes. Furthermore, pro-B cells are frequently located in the vicinity of HSCs in the same niche. Using an interactome bioinformatics pipeline, we identify Nidogen-1 as essential for pro-B cell retention in the peri-sinusoidal niche as confirmed in Nidogen-1-/- mice. Finally, human pro-B cells and hematopoietic progenitors are observed close to similar IL-7+ stromal cells. Thus, a multispecific niche exists in mouse and human supporting both early progenitors and committed hematopoietic lineages.

KEYWORDS:

B cell development; bone marrow; hematopoietic stem cell; interaction network; stromal cell niche

PMID:
30893599
DOI:
10.1016/j.celrep.2019.02.065
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