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ACS Appl Mater Interfaces. 2019 May 1;11(17):16198-16206. doi: 10.1021/acsami.9b02005. Epub 2019 Apr 17.

Ultrasensitive Microfluidic Paper-Based Electrochemical Biosensor Based on Molecularly Imprinted Film and Boronate Affinity Sandwich Assay for Glycoprotein Detection.

Sun X1,2, Jian Y1,2, Wang H1,2, Ge S1, Yan M2, Yu J2.

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Shandong Collaborative Innovation Center of Technology and Equipements for Biological Diagnosis and Therapy, Institute for Advanced Interdisciplinary Research , University of Jinan , Jinan 250022 , P.R. China.
School of Chemistry and Chemical Engineering , University of Jinan , Jinan 250022 , P.R. China.


In this work, we proposed a strategy that combined molecularly imprinted polymers (MIPs) and hybridization chain reaction into microfluidic paper-based analytical devices for ultrasensitive detection of target glycoprotein ovalbumin (OVA). During the fabrication, Au nanorods with a large surface area and superior conductibility were grown on paper cellulosic fiber as a matrix to introduce a boronate affinity sandwich assay. The composite of MIPs including 4-mercaptophenylboronic acid (MPBA) was able to capture target glycoprotein OVA. SiO2@Au nanocomposites labeled MPBA and cerium dioxide (CeO2)-modified nicked DNA double-strand polymers (SiO2@Au/dsDNA/CeO2) as a signal tag were captured into the surface of the electrode in the presence of OVA. An electrochemical signal was generated by using nanoceria as redox-active catalytic amplifiers in the presence of 1-naphthol in electrochemical assays. As a result, the electrochemical assay was fabricated and could be applied in the detection of OVA in the wide linear range of 1 pg/mL to 1000 ng/mL with a relatively low detection limit of 0.87 pg/mL (S/N = 3). The results indicated that the proposed platform possessed potential applications in clinical diagnosis and other related fields.


boronate affinity sandwich assay; electrochemical sensor; glycoprotein; hybridization chain reaction; molecularly imprinted film


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