Format

Send to

Choose Destination
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:511-519. doi: 10.1016/j.msec.2019.02.001. Epub 2019 Feb 1.

Fabrication of electrochemical biosensor consisted of multi-functional DNA structure/porous au nanoparticle for avian influenza virus (H5N1) in chicken serum.

Author information

1
Department of Chemical Engineering, Wolgye-dong, Nowon-gu, Seoul 01899, Republic of Korea. Electronic address: tlee@kw.ac.kr.
2
Department of Chemical Engineering, Wolgye-dong, Nowon-gu, Seoul 01899, Republic of Korea.
3
Department of Chemistry, Kwangwoon University, Wolgye-dong, Nowon-gu, Seoul 01899, Republic of Korea.
4
School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 06910, Republic of Korea.
5
School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 06910, Republic of Korea. Electronic address: mhlee7@cau.ac.kr.
6
School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 06910, Republic of Korea. Electronic address: junmin@cau.ac.kr.

Abstract

Avian influenza virus (AIV) is one of the most harmful pathogens to living things due to its fast infection, various mutations, and dangerous symptoms. In this study, we fabricated a label-free AIV H5N1 biosensor composed of multi-functional DNA structure on a porous Au nanoparticles (pAuNPs) fabricated electrode using the electrochemical (EC) technique. As a multi-functional bioprobe, the DNA 3 way-junction (3WJ) was introduced. Each fragment of DNA 3WJ was rolled to recognition part (hemagglutinin (HA) protein detection aptamer), EC signal generation part (horseradish peroxidase (HRP)-mimicked DNAzyme), and immobilization part (Thiol group). Each fragment was assembled in order to form the DNA 3WJ for AI detection and the assembled structure was confirmed by native-tris boric acid magnesium polyacrylamide gel electrophoresis (TBM-PAGE). Moreover, in order to increase the electrochemical signal sensitivity, pAuNPs were synthesized. The property of pAuNPs was investigated by field emission scanning electron microscopy (FE-SEM), Transmission electron microscopy (TEM), Ultraviolet-visible (UV-VIS) spectroscopy and zeta potential analysis. The DNA 3WJ on pAuNPs-modified Au electrode was then prepared using the layer-by-layer (LbL) assembly method. FE-SEM and atomic force microscopy (AFM) were used to investigate the surface morphology. Cyclic voltammetry (CV) was carried out to confirm the HA protein binding to DNA 3WJ-modified electrode. Moreover, The HA protein can be detected 1 pM in HEPES solution and 1 pM in diluted-chicken serum, respectively. The present study showed label-free, simple fabrication, and easy-to-tailor detection elements for AIV. The present biosensor can be a powerful candidate for various virus detection platforms.

KEYWORDS:

Avian influenza virus detection; DNA 3 way-junction; Electrochemical biosensor; Hemagglutinin; Multi-functional DNA structure; Porous Au nanoparticle

PMID:
30889726
DOI:
10.1016/j.msec.2019.02.001

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center