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Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1459-1468. doi: 10.1016/j.msec.2019.02.047. Epub 2019 Feb 16.

Graphene oxide functionalized with chitosan based nanoparticles as a carrier of siRNA in regulating Bcl-2 expression on Saos-2 & MG-63 cancer cells and its inflammatory response on bone marrow derived cells from mice.

Author information

1
Department of Chemical Engineering, Anna University, Chennai 600025, India; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen 4010, Hungary.
2
Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Incheon 22212, Republic of Korea.
3
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen 4010, Hungary.
4
Department of Orthodontics, Saveetha Dental College, Chennai 600077, India.
5
SRM University, Kattankulathur, Tamil Nadu 603203, India.
6
Department of Chemical Engineering, Anna University, Chennai 600025, India.
7
Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, Incheon 22212, Republic of Korea. Electronic address: yunsuk.huh@inha.ac.kr.
8
Department of Chemical Engineering, Anna University, Chennai 600025, India. Electronic address: nbsbala@annauniv.edu.

Abstract

Presently, quite a lot of research that are being carried out to find a potential cure for cancer and many had made to clinical trial stage as well. In the present study, we focus on use of a novel graphene oxide functionalized chitosan nanoparticle targeting Saos-2 and MG-63 osteosarcoma cells. The graphene oxide chitosan nanoparticles were loaded with siRNA, studied for in vitro release with varying concentration & pH, and fitted to peppas model. MTT & ROS assay was used to evaluate biocompatibility of carrier and qPCR to study the inflammatory responses in particular checking gene expression of IL-6, TGF-ß, TNF-α in both RAW 264.7 and bone marrow derived macrophages. The results of study showed that release of siRNA were in a controlled fashion and effective at acidic pH that prevails on tumor site. The material was biocompatible and effective in case of Saos-2 osteosarcoma cells with a viability of 0.4 ± 0.43 and 0.49 ± 0.53 in case of MG-63 cells when treated with highest concentration of 100 μl siRNA compared to untreated cells that were in range of 0.64 ± 0.67 in Saos-2 and 0.61 ± 0.63 in MG-63 cells. The results of expression of inflammatory cytokines IL-6, TGF-β & TNF-α showed negligible amount compared to control group serving the purpose of an effective carrier targeting tumor cells.

KEYWORDS:

Bone marrow derived macrophages; Chitosan; Graphene oxide; Inflammation; Osteosarcoma; RAW macrophages; siRNA

PMID:
30889680
DOI:
10.1016/j.msec.2019.02.047
[Indexed for MEDLINE]

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